Rabbit anti-Human NQO1 Polyclonal Antibody | anti-NQO1 antibody

NQO1 (NAD(P)H Dehydrogenase Quinone 1, Azoreductase, Cytochrome b-5 Reductase, DHQU, Diaphorase (NADH/NADPH) (Cytochrome b-5 Reductase), Diaphorase 4, DIA4, Dioxin Inducible 1, DT Diaphorase, DTD, Menadione Reductase, NAD(P)H Menadione Oxidoreductase 1 Di

Cat. Num. AAA6490446

• Gene Names: NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: NQO1; Polyclonal Antibody; NQO1 (NAD(P)H Dehydrogenase Quinone 1; Azoreductase; Cytochrome b-5 Reductase; DHQU; Diaphorase (NADH/NADPH) (Cytochrome b-5 Reductase); Diaphorase 4; DIA4; Dioxin Inducible 1; DT Diaphorase; DTD; Menadione Reductase; NAD(P)H Menadione Oxidoreductase 1 Di; NAD(P)H Dehydrogenase [Quinone] 1; EC=1.6.5.2; anti-NQO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NQO1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-NQO1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human NQO1 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NQO1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(NQO1 antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NQO1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-NQO1 antibody

Antibodies; Enzymes; Cytochrome

References

1. Hubackova, M., Vaclavikova, R. Pharmacogenet. Genomics 19(7), 505-512 (2009). 2. Canova, C., Hashibe, M. Cancer Res. 69(7), 2956-2965 (2009). 3. Li, Y. and Jaiswal, A.K. J. Biol. Chem. 267(21), 15097-15104 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 118607
• NCBI GeneID: 1728
• UniProt Accession #: P15559
• Molecular Weight: 33.0 kDa (294aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: NAD(P)H dehydrogenase
• NCBI Official Synonym Full Names: NAD(P)H quinone dehydrogenase 1
• NCBI Official Symbol: NQO1
• NCBI Official Synonym Symbols: DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• NCBI Protein Information: NAD(P)H dehydrogenase [quinone] 1
• UniProt Protein Name: NAD(P)H dehydrogenase [quinone] 1
• Protein Family: NAD(P)H dehydrogenase
• UniProt Gene Name: NQO1
• UniProt Synonym Gene Names: DIA4; NMOR1; DTD; QR1
• UniProt Entry Name: NQO1_HUMAN

NCBI Description

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer's disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.6.5.2

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell soma; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; NAD(P)H dehydrogenase (quinone) activity; cytochrome-b5 reductase activity; superoxide dismutase activity

Biological Process: response to ethanol; removal of superoxide radicals; response to toxin; xenobiotic metabolic process; positive regulation of neuron apoptosis; negative regulation of catalytic activity; regulation of amino acid metabolic process; nitric oxide biosynthetic process; response to nutrient; response to estradiol stimulus; aging; synaptic transmission, cholinergic