Rabbit NQO1 Polyclonal Antibody | anti-NQO1 antibody

NQO1 Rabbit Polyclonal

Cat. Num. AAA7602063

• Gene Names: NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: NQO1; Polyclonal Antibody; NQO1 Rabbit Polyclonal; anti-NQO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 202

• Applicable Applications for anti-NQO1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Immunogen: NAD(P)H dehydrogenase, quinone 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using MBS7602063 (NQO1 Antibody) at dilution of 1:200)

Western Blot (WB)

(K-562 cells were subjected to SDS PAGE followed by western blot with MBS7602063 (NQO1 Antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 554790420
• NCBI GeneID: 1728
• NCBI Accession #: NP_001273066.1
• NCBI GenBank Nucleotide #: NM_001286137.1
• Molecular Weight: 26,365 Da
• NCBI Official Full Name: NAD(P)H dehydrogenase
• NCBI Official Synonym Full Names: NAD(P)H quinone dehydrogenase 1
• NCBI Official Symbol: NQO1
• NCBI Official Synonym Symbols: DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• NCBI Protein Information: NAD(P)H dehydrogenase [quinone] 1
• UniProt Protein Name: NAD(P)H dehydrogenase [quinone] 1
• Protein Family: NAD(P)H dehydrogenase
• UniProt Gene Name: NQO1
• UniProt Synonym Gene Names: DIA4; NMOR1; DTD; QR1
• UniProt Entry Name: NQO1_HUMAN

NCBI Description

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer's disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.6.5.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell soma; cytoplasm; cytosol

Molecular Function: cytochrome-b5 reductase activity; identical protein binding; NAD(P)H dehydrogenase (quinone) activity; protein binding; superoxide dismutase activity

Biological Process: aging; negative regulation of catalytic activity; nitric oxide biosynthetic process; positive regulation of neuron apoptosis; regulation of amino acid metabolic process; removal of superoxide radicals; response to estradiol stimulus; response to ethanol; response to nutrient; response to toxin; synaptic transmission, cholinergic; xenobiotic metabolic process