Rabbit anti-Human NPFF Polyclonal Antibody | anti-NPFF antibody

NPFF, CT (NPFF, FMRFamide-related peptides, Neuropeptide SF, Neuropeptide FF, Neuropeptide AF) (Azide free) (HRP)

Cat. Num. AAA6326219

• Gene Names: NPFF; FMRFAL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NPFF; Polyclonal Antibody; CT (NPFF; FMRFamide-related peptides; Neuropeptide SF; Neuropeptide FF; Neuropeptide AF) (Azide free) (HRP); Neuropeptide AF; anti-NPFF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NPFF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NPFF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-113 amino acids from the C-terminal region of human NPFF.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NPFF Antibody (C-term) in 293 cell line lysates (35ug/lane).NPFF (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(NPFF Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NPFF antibody

FMFRamide-related protein precursor plays a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. FMRFAL encodes a preproprotein which is cleaved to form two active peptides with similar function.

Product Categories/Family for anti-NPFF antibody

Antibodies; Neuroscience

References

Yang, H.Y., et al. Neuropeptides 42(1):1-18(2008). Roumy, M., et al. J. Biol. Chem. 282(11):8332-8342(2007). Anko, M.L., et al. J. Neurochem. 96(2):573-584(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 8134595
• NCBI GeneID: 8620
• UniProt Accession #: O15130; Q3SXL4
• Molecular Weight: 113
• NCBI Official Full Name: Pro-FMRFamide-related neuropeptide FF
• NCBI Official Synonym Full Names: neuropeptide FF-amide peptide precursor
• NCBI Official Symbol: NPFF
• NCBI Official Synonym Symbols: FMRFAL
• NCBI Protein Information: pro-FMRFamide-related neuropeptide FF
• UniProt Protein Name: Pro-FMRFamide-related neuropeptide FF
• Protein Family: Pro-FMRFamide-related neuropeptide
• UniProt Gene Name: NPFF
• UniProt Synonym Gene Names: NPSF; NPFF; NPAF
• UniProt Entry Name: NPFF_HUMAN

NCBI Description

FMFRamide-related protein precursor plays a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. FMRFAL encodes a preproprotein which is cleaved to form two active peptides with similar function. [provided by RefSeq, Jul 2008]

Uniprot Description

NPFF: Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels. Belongs to the FARP (FMRFamide related peptide) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular space; dendrite; extracellular region; perikaryon; nerve terminal; vesicle

Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding; receptor binding

Biological Process: response to drug; negative regulation of heart rate; maternal process involved in pregnancy; response to morphine; negative regulation of appetite; negative regulation of insulin secretion; vasopressin secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spinal cord development; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; positive regulation of blood pressure; acute inflammatory response to antigenic stimulus