Rabbit anti-Human NPC1L1 Polyclonal Antibody | anti-NPC1L1 antibody

NPC1L1, NT (NPC1L1, Niemann-Pick C1-like protein 1) (AP)

Cat. Num. AAA6326204

• Gene Names: NPC1L1; NPC11L1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NPC1L1; Polyclonal Antibody; NT (NPC1L1; Niemann-Pick C1-like protein 1) (AP); Niemann-Pick C1-like protein 1; anti-NPC1L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NPC1L1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NPC1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the N-terminal region of human NPC1L1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NPC1L1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NPC1L1 antibody detected the NPC1L1 protein (arrow).)

Related Product Information for anti-NPC1L1 antibody

The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-NPC1L1 antibody

Antibodies; Lipoproteins

References

Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Teslovich, T.M., et al. Nature 466(7307):707-713(2010). Pramfalk, C., et al. J. Lipid Res. 51(6):1354-1362(2010). Maeda, T., et al. J. Atheroscler. Thromb. 17(4):356-360(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 156231351
• NCBI GeneID: 29881
• NCBI Accession #: NP_037521.2
• NCBI GenBank Nucleotide #: NM_013389.2
• UniProt Accession #: Q9UHC9; Q17RV5; Q6R3Q4; Q9UHC8; A4D2J7; D3DVK9
• Molecular Weight: 140kD
• NCBI Official Full Name: Niemann-Pick C1-like protein 1 isoform 1
• NCBI Official Synonym Full Names: NPC1-like 1
• NCBI Official Symbol: NPC1L1
• NCBI Official Synonym Symbols: NPC11L1
• NCBI Protein Information: Niemann-Pick C1-like protein 1; NPC1 (Niemann-Pick disease, type C1, gene)-like 1
• UniProt Protein Name: Niemann-Pick C1-like protein 1
• Protein Family: Niemann-Pick C1-like protein
• UniProt Gene Name: NPC1L1

NCBI Description

The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

NPC1L1: Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Interacts with RAB11A, MYO5B and RAB11FIP2. Interaction with RAB11A, MYO5B and RAB11FIP2 is required for proper transport to the plasma membrane upon cholesterol depletion. Expression is decreased in Caco-2 cells upon PPARD activation. Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. Belongs to the patched family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: apical plasma membrane; brush border membrane; cytoplasmic vesicle membrane; integral component of membrane; plasma membrane

Molecular Function: drug binding; myosin V binding; protein binding; Rab GTPase binding

Biological Process: cholesterol biosynthetic process; cholesterol transport; intestinal cholesterol absorption; lipoprotein metabolic process; response to drug