SDS-Page
HSD17B14 recombinant protein
HSD17B14, 1-270aa, Human, His tag, E Coli
Gene Names
HSD17B14; DHRS10; SDR47C1; retSDR3
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
HSD17B14; 1-270aa; Human; His tag; E Coli; 17-beta-hydroxysteroid dehydrogenase 14; DHRS10; retSDR3; SDR47C1; ; HSD17B14 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Concentration
1.0 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATG TRYAGKVVVV TGGGRGIGAG IVRAFVNSGA RVVICDKDES GGRALEQELP GAVFILCDVT QEDDVKTLVS ETIRRFGRLD CVVNNAGHHP PPQRPEETSA QGFRQLLELN LLGTYTLTKL ALPYLRKSQG NVINISSLVG AIGQAQAVPY VATKGAVTAM TKALALDESP YGVRVNCISP GNIWTPLWEE LAALMPDPRA TIREGMLAQP LGRMGQPAEV GAAAVFLASE ANFCTGIELL VTGGAELGYG CKASRSTPVD APDIPS
Sequence Length
270
Applicable Applications for HSD17B14 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for HSD17B14 recombinant protein
HSD17B14, also known as 17-beta-hydroxysteroid dehydrogenase 14, belongs to the 17-beta-HSD family of proteins, which regulate the availability of steroids within various tissues rough out the body. It exists as a homotetramer that localizes to the cytoplasm and is highly expressed in brain, placenta, liver and kidney. Recombinant human HSD17B14 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for HSD17B14 recombinant protein
References
Grimwood J., et al. (2004), Nature., 428: 529-535.; Lukacik P., et al. (2007). Biochem. J., 402: 419-427
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32.4 kDa (306aa) confirmed by MALDI-TOF
NCBI Official Full Name
17-beta-hydroxysteroid dehydrogenase 14
NCBI Official Synonym Full Names
hydroxysteroid 17-beta dehydrogenase 14
NCBI Official Symbol
HSD17B14
NCBI Official Synonym Symbols
DHRS10; SDR47C1; retSDR3
NCBI Protein Information
17-beta-hydroxysteroid dehydrogenase 14
UniProt Protein Name
17-beta-hydroxysteroid dehydrogenase 14
Protein Family
UniProt Gene Name
HSD17B14
UniProt Synonym Gene Names
DHRS10; SDR3; SDR47C1; 17-beta-HSD 14
NCBI Description
17-beta-hydroxysteroid dehydrogenases, such as HSD17B14, are primarily involved in metabolism of steroids at the C17 position and also of other substrates, such as fatty acids, prostaglandins, and xenobiotics (Lukacik et al., 2007 [PubMed 17067289]).[supplied by OMIM, Jun 2009]
Uniprot Description
Has NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity. Converts oestradiol to oestrone. The physiological substrate is not known. Acts on oestradiol and 5-androstene-3-beta,17-beta-diol (in vitro).
Research Articles on HSD17B14
Similar Products
Product Notes
The HSD17B14 hsd17b14 (Catalog #AAA203849) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's HSD17B14 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HSD17B14 hsd17b14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATG TRYAGKVVVV TGGGRGIGAG IVRAFVNSGA RVVICDKDES GGRALEQELP GAVFILCDVT QEDDVKTLVS ETIRRFGRLD CVVNNAGHHP PPQRPEETSA QGFRQLLELN LLGTYTLTKL ALPYLRKSQG NVINISSLVG AIGQAQAVPY VATKGAVTAM TKALALDESP YGVRVNCISP GNIWTPLWEE LAALMPDPRA TIREGMLAQP LGRMGQPAEV GAAAVFLASE ANFCTGIELL VTGGAELGYG CKASRSTPVD APDIPS. It is sometimes possible for the material contained within the vial of "HSD17B14, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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