Western Blot (WB)
(Western Blot (WB) analysis of NPAS4 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate)
Rabbit anti-Human, Mouse NPAS4 Polyclonal Antibody | anti-NPAS4 antibody
NPAS4 Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NPAS4; Polyclonal Antibody; NPAS4 Polyclonal Antibody; Neuronal PAS domain-containing protein 4; Neuronal PAS4; Class E basic helix-loop-helix protein 79; bHLHe79; HLH-PAS transcription factor NXF; PAS domain-containing protein 10; BHLHE79; NXF; PASD10; anti-NPAS4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
NPAS4 polyclonal antibody detects endogenous levels of NPAS4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
802
Applicable Applications for anti-NPAS4 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human NPAS4.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of NPAS4 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of NPAS4 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate)
Related Product Information for anti-NPAS4 antibody
The Per-Arnt-Sim (PAS) domain is a 270 amino acid motif that mediates associations among various PAS family transcription factors. The PAS family contains neuronal specific transcription factors known as NPAS1, NPAS2, NPAS3 and NPAS4, which are involved in the development and maintenance of learning and memory pathways. NPAS1 regulates erythropoietin expression in developing brain. NPAS2, also designated PAS 4/MOP4, associates with MOP3 to activate transcription. NPAS3, which localizes to the nucleus and is ubiquitously expressed in the adult brain, may be involved in neurogenesis and has been implied to control regulatory pathways relevant to psychotic illness and schizophrenia. NPAS4 (neuronal PAS domain protein 4), also known as NXF, Le-PAS, PASD10 or bHLHe79, is a 802 amino acid nuclear protein that is exclusively expressed in the brain. NPAS4 acts as a transcriptional activator in the presence of Arnt 1, and activates the CME (CNS midline enhancer) element and expression of the Drebrin gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 87kDa
NCBI Official Full Name
neuronal PAS domain-containing protein 4 isoform 1
UniProt Protein Name
Neuronal PAS domain-containing protein 4
Protein Family
UniProt Gene Name
NPAS4
UniProt Synonym Gene Names
BHLHE79; NXF; PASD10; Neuronal PAS4; bHLHe79
UniProt Entry Name
NPAS4_HUMAN
Uniprot Description
NPAS4: Acts as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: transcription factor complex; nucleus
Molecular Function: signal transducer activity; protein heterodimerization activity
Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; signal transduction
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Product Notes
The NPAS4 npas4 (Catalog #AAA3007272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPAS4 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NPAS4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the NPAS4 npas4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPAS4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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