Mouse NOXA Monoclonal Antibody | anti-NOXA antibody

NOXA Monoclonal Antibody

Cat. Num. AAA668043

• Gene Names: PMAIP1; APR; NOXA
• Reactivity: Human (Predicted Reactivity: Mouse
• Applications: Western Blot
• Purity: Protein G Chromatography
• Synonyms: NOXA; Monoclonal Antibody; NOXA Monoclonal Antibody; anti-NOXA antibody

• Host: Mouse
• Reactivity: Human (Predicted Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: ABM16G6
• Purity/Purification: Protein G Chromatography
• Form/Format: 100 ug in 200 ul PBS containing 0.05'\0 BSA and 005% sodium azide. Sodium azide is highly toxic.
• Sequence Length: 54

• Applicable Applications for anti-NOXA antibody: Western Blot (WB)
• Application Notes: Western Blot Analysis: 2-4ug/ml
• Antigen: NOXA
• Immunogen: A full length recombinant protein was used as the immunogen for this antibody.
• Preparation and Storage: Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire 1 year from date of purchase.

Western Blot (WB)

(Expression analysis of NOXA. Anti- NOXA antibody (Clone:ABM16G6) was tested at 2 µg/ml on Molt4 Lysate)

Related Product Information for anti-NOXA antibody

Noxa IS a BH3-only pro-apoptotic protein that mediates apoptosis by specifically inhibiting the anti-apoptotic Bcl-2 family member Mcl-1. It consists of 54 amino aCids with one BH3 domain. The gene for NOXA is a target of p53. In response to cellular stress Noxa is induced by p53 and mediates p53-induced apoptosis But more recently, it has been shown that NOXA can also be induced independently of p53 by other transcription factors such as p73 and E2F1. Noxa is also found to be induced by hypoxia-inducible factor (HIF-1) and mediates HIF-1 induced hypoxic cell death. Noxa is commonly upregulated in melanomas. and is associated with melanoma development and progression. Increase in Noxa expression is driven by oncogenic activation of MEK/ERK signaling through the transcription factor CREB (cAMP responsive element binding protein).

Product Categories/Family for anti-NOXA antibody

Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 10863923
• NCBI GeneID: 5366
• NCBI Accession #: NP_066950.1
• NCBI GenBank Nucleotide #: NM_021127.2
• UniProt Accession #: Q13794; Q8N589; B2R4T7
• Molecular Weight: 14,955 Da
• NCBI Official Full Name: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Synonym Full Names: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Symbol: PMAIP1
• NCBI Official Synonym Symbols: APR; NOXA
• NCBI Protein Information: phorbol-12-myristate-13-acetate-induced protein 1; PMA-induced protein 1; adult T cell leukemia-derived PMA-responsive; immediate-early-response protein APR; protein Noxa
• UniProt Protein Name: Phorbol-12-myristate-13-acetate-induced protein 1
• Protein Family: Superoxide-generating NADPH oxidase
• UniProt Gene Name: PMAIP1
• UniProt Synonym Gene Names: NOXA; PMA-induced protein 1
• UniProt Entry Name: APR_HUMAN

Uniprot Description

NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 18q21.32

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; nucleus

Molecular Function: protein binding

Biological Process: caspase activation; proteasomal protein catabolic process; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; response to dsRNA; positive regulation of caspase activity; cellular response to glucose starvation; positive regulation of protein oligomerization; negative regulation of fibroblast proliferation; positive regulation of neuron apoptosis; T cell homeostasis; positive regulation of DNA damage response, signal transduction by p53 class mediator; regulation of mitochondrial membrane permeability; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to X-ray; response to UV