Immunohistochemistry (IHC)
(Anti-NOTCH2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
Rabbit NOTCH2 / NOTCH-2 Polyclonal Antibody | anti-NOTCH2 antibody
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
Immunohistochemistry (IHC)
(Anti-NOTCH2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
Immunohistochemistry (IHC)
(Anti-NOTCH2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
Western Blot (WB)
(Western blot of anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (u)
Western Blot (WB)
(Western blot of anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (u)
Western Blot (WB)
(Anti-Notch 2 Antibody - Western Blot. Western blot of anti-Notch 2 (intra) antibody shows detection of a band at ~110 kD corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100 ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.)
Western Blot (WB)
(Anti-Notch 2 Antibody - Western Blot. Western blot of anti-Notch 2 (intra) antibody shows detection of a band at ~110 kD corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100 ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Uniprot Description
Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.
Protein type: Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1p13-p11
Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; membrane; integral to plasma membrane; plasma membrane; extracellular region; nucleus; receptor complex; cilium
Molecular Function: protein binding; receptor activity; calcium ion binding
Biological Process: apoptosis; positive regulation of apoptosis; multicellular organismal development; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; bone remodeling; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; morphogenesis of an epithelial sheet; hemopoiesis; cell growth; cell cycle arrest; positive regulation of BMP signaling pathway; embryonic limb morphogenesis; nervous system development; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; intracellular receptor-mediated signaling pathway; in utero embryonic development; stem cell maintenance; Notch receptor processing; humoral immune response; organ morphogenesis; inflammatory response to antigenic stimulus; defense response to bacterium; gene expression; positive regulation of Ras protein signal transduction; determination of left/right symmetry; negative regulation of apoptosis
Disease: Hajdu-cheney Syndrome; Alagille Syndrome 2
Research Articles on NOTCH2
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Product Notes
The NOTCH2 notch2 (Catalog #AAA240540) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human NOTCH2 / NOTCH-2 reacts with Chimpanzee, Dog, Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOTCH2 / NOTCH-2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA (1:30000 - 1:90000), IF, IHC-P (1:500), WB (1:400 - 1:2000) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the NOTCH2 notch2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOTCH2 / NOTCH-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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