Rabbit anti-Mouse NOS-1 Polyclonal Antibody | anti-NOS-1 antibody

NOS-1 Rabbit pAb

Cat. Num. AAA4754955

• Gene Names: NANOS1; NOS1; NOS-1; SPGF12; EC_Rep1a; ZC2HC12A
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: NOS-1; Polyclonal Antibody; NOS-1 Rabbit pAb; NOS1; SPGF12; anti-NOS-1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-NOS-1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Synthetic peptide of human NANOS1
• Conjugation: Unconjugated
• Molecular Function: Nanos1, also known as NOS1, is a 292 amino acid protein that localizes to the perinuclear region of the cytoplasm and contains one nanos-type zinc finger. Expressed at high levels in spermatogonia and present at lower levels in fetal ovaries, Nanos1 forms a complex with Pumilio 2 and functions to regulate the translation of select mRNAs, specifically via association with the 3’-UTR of its mRNA targets. Additionally, Nanos1 is required for the establishment and maintenance of germline stem cells, as it prevents their premature entry into oogenesis.
• Modification: Unmodified
• Cell Position: Cytoplasm
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse muscle tissue, Primary antibody: 162847(NANOS1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)

Product Categories/Family for anti-NOS-1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 40804761
• NCBI GeneID: 340719
• NCBI Accession #: NP_955631
• NCBI GenBank Nucleotide #: NM_199461
• UniProt Accession #: Q8WY41
• Molecular Weight: 32kDa
• NCBI Official Full Name: nanos homolog 1
• NCBI Official Synonym Full Names: nanos C2HC-type zinc finger 1
• NCBI Official Symbol: NANOS1
• NCBI Official Synonym Symbols: NOS1; NOS-1; SPGF12; EC_Rep1a; ZC2HC12A
• NCBI Protein Information: nanos homolog 1
• UniProt Protein Name: Nanos homolog 1
• UniProt Gene Name: NANOS1
• UniProt Synonym Gene Names: NOS1; NOS-1
• UniProt Entry Name: NANO1_HUMAN

NCBI Description

This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]

Uniprot Description

NANOS1: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. Belongs to the nanos family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding; translation repressor activity; zinc ion binding; RNA binding

Biological Process: cell migration; negative regulation of translation

Disease: Spermatogenic Failure 12