Rabbit Nogo B receptor Polyclonal Antibody | anti-NUS1 antibody

Nogo B receptor Rabbit pAb

Cat. Num. AAA4752128

• Gene Names: NUS1; NgBR; C6orf68; TANGO14; MGC:7199
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Nogo B receptor; Polyclonal Antibody; Nogo B receptor Rabbit pAb; Dehydrodolichyl diphosphate synthase complex subunit NUS1; NgBR; MRD55; CDG1AA; C6orf68; TANGO14; MGC:7199; anti-NUS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-NUS1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen: A synthetic peptide of human Nogo B receptor
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of Nogo B receptor in K562,3T3,Hela cell lysates using Nogo B receptor Rabbit pAb(1:1000 diluted). Predicted band size:33kDa. Observed band size:33kDa.)

Related Product Information for anti-NUS1 antibody

This gene encodes a type I single transmembrane domain receptor, which is a subunit of cis-prenyltransferase, and serves as a specific receptor for the neural and cardiovascular regulator Nogo-B. The encoded protein is essential for dolichol synthesis and protein glycosylation. This gene is highly expressed in non-small cell lung carcinomas as well as estrogen receptor-alpha positive breast cancer cells where it promotes epithelial mesenchymal transition. This gene is associated with the poor prognosis of human hepatocellular carcinoma patients. Naturally occurring mutations in this gene cause a congenital disorder of glycosylation and are associated with epilepsy. A knockout of the orthologous gene in mice causes embryonic lethality before day 6.5. Pseudogenes of this gene have been defined on chromosomes 13 and X. [provided by RefSeq, May 2017]
With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER) (PubMed:21572394, PubMed:25066056, PubMed:28842490). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator (PubMed:16835300).

NCBI and Uniprot Product Information

• NCBI GI #: 20270243
• NCBI GeneID: 116150
• NCBI Accession #: NP_612468.1
• NCBI GenBank Nucleotide #: NM_138459.3
• UniProt Accession #: Q96E22; O00251; B2RWQ4
• Molecular Weight: 33,224 Da
• NCBI Official Full Name: nogo-B receptor
• NCBI Official Synonym Full Names: nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae)
• NCBI Official Symbol: NUS1
• NCBI Official Synonym Symbols: NgBR; C6orf68; TANGO14; MGC:7199
• NCBI Protein Information: nogo-B receptor; transport and golgi organization 14 homolog
• UniProt Protein Name: Nogo-B receptor
• Protein Family: Dehydrodolichyl diphosphate synthase complex
• UniProt Gene Name: NUS1
• UniProt Synonym Gene Names: C6orf68; NGBR; NgBR
• UniProt Entry Name: NGBR_HUMAN

Uniprot Description

Function: Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator. Regulates the stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Ref.5 Ref.7Essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Ref.5 Ref.7

Pathway: Protein modification; protein glycosylation.

Subunit structure: Interacts with DHDDS, promoting its isoprenyltransferase activity. Interacts with NPC2. Ref.6 Ref.7

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note: Colocalizes with Nogo-B during VEGF and wound healing angiogenesis. Ref.6 Ref.7

Miscellaneous: Although strongly related to UPP synthase family proteins, it has no lipid transferase activity.NUS1 seems to exist in two topological orientations, a minor glycosylated species with its C-terminus oriented towards the lumen regulating NPC2 stability, and a major fraction oriented with its C-terminus directed towards the cytosol where it regulates cis-IPTase activity.

Sequence similarities: Belongs to the UPP synthase family.

Sequence caution: The sequence AAB72234.1 differs from that shown. Reason: Frameshift at several positions.