Western Blot (WB)
(Western Blot analysis of Nogo on mouse skeletal muscle tissue (1) and Hela cell (2) lysate using anti-Nogo antibody at 1/1,000 dilution.)
Rabbit anti-Human, Mouse Nogo B Polyclonal Antibody | anti-NogoB antibody
Nogo B Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Nogo B; Polyclonal Antibody; Nogo B Polyclonal Antibody; Nogo protein; NSP; RTN4; KIAA0886; NOGO; My043; SP1507; 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; Foocen; Glut4 vesicle 20 kDa protein; Human NogoA; Kiaa0886; KIAA4153; MGC116054; MGC139261; mKIAA0886; mKIAA4153; My043 protein; Nbla00271; Nbla10545; Neurite growth inhibitor 220; Neurite Growth Inhibitor 220; included; Neurite outgrowth inhibitor; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog; NI-250; NI220/250; Nogo A; Nogo C; NOGOC; NSP-CL; rat N; Reticulon 4; Reticulon 5; Reticulon-4; Reticulon-5; RTN X; RTN-x; Rtn4; Rtn4 reticulon 4; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; Vp20; anti-NogoB antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Nogo B polyclonal antibody detects endogenous levels of Nogo B protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-NogoB antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2,000
ICC: 1:50-1:200
IF: 1:50-1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IP: 1:10-1:50
ICC: 1:50-1:200
IF: 1:50-1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IP: 1:10-1:50
Immunogen
Recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Nogo on mouse skeletal muscle tissue (1) and Hela cell (2) lysate using anti-Nogo antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of Nogo on mouse skeletal muscle tissue (1) and Hela cell (2) lysate using anti-Nogo antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining Nogo in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Nogo in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-NogoB antibody
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
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Product Notes
The NogoB (Catalog #AAA3013139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nogo B Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nogo B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 ICC: 1:50-1:200 IF: 1:50-1:100 IHC: 1:50-1:200 FC/FACS: 1:50-1:100 IP: 1:10-1:50. Researchers should empirically determine the suitability of the NogoB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nogo B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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