Rabbit anti-Human, Mouse NOD4 Polyclonal Antibody | anti-NLRC5 antibody

NOD4 Antibody

Cat. Num. AAA151554

• Gene Names: NLRC5; NOD4; NOD27; CLR16.1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: NOD4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: NOD4; Polyclonal Antibody; NOD4 Antibody; NOD27; CLR16.1; Protein NLRC5; Nucleotide-binding oligomerization domain protein 27; NLR family; CARD domain containing 5; anti-NLRC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NOD4 antibody is predicted to not cross-react with other members of the NOD family of proteins.
• Purity/Purification: NOD4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1866

• Applicable Applications for anti-NLRC5 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: NOD4 antibody can be used for detection of NOD4 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NOD4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NOD4.
• Buffer: NOD4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NOD4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NOD4 in EL4 cell lysate with NOD4 antibody at 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of NOD4 in HeLa cells with NOD4 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NOD4 in HeLa cells with NOD4 antibody at 20 μg/mL.)

Related Product Information for anti-NLRC5 antibody

NOD4 Antibody: NOD4 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD4 contains a CARD-like domain, a central NOD domain and a large LRR region. NOD4, an IFN-gamma-inducible nuclear protein, plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. As a key negative regulator of NF-κB and type I interferon signaling, NOD4 may be a useful target for manipulating immune responses against infectious or inflammation-associated diseases, including cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 116268109
• NCBI GeneID: 84166
• NCBI Accession #: NP_115582
• NCBI GenBank Nucleotide #: NM_032206.3
• UniProt Accession #: Q86WI3; Q6P4A6; Q86VM7; Q8NF42; Q8TEE2; Q8TEJ1; Q969L7; B5MEF1; C9JMD8
• Molecular Weight: 205 kDa
• NCBI Official Full Name: protein NLRC5
• NCBI Official Synonym Full Names: NLR family, CARD domain containing 5
• NCBI Official Symbol: NLRC5
• NCBI Official Synonym Symbols: NOD4; NOD27; CLR16.1
• NCBI Protein Information: protein NLRC5; NOD-like receptor C5; caterpiller protein 16.1; nucleotide-binding oligomerization domains 27; nucleotide-binding oligomerization domain protein 4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 5
• UniProt Protein Name: Protein NLRC5
• Protein Family: Protein
• UniProt Gene Name: NLRC5
• UniProt Synonym Gene Names: NOD27; NOD4; CLR16.1
• UniProt Entry Name: NLRC5_HUMAN

NCBI Description

This gene encodes a member of the caspase recruitment domain-containing NLR family. This gene plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways. [provided by RefSeq, Oct 2011]

Uniprot Description

NLRC5: Probable regulator of the NF-kappa-B and type I interferon signaling pathways. May also regulate the type II interferon signaling pathway. Plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. Belongs to the NLRP family. 6 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; ATP binding

Biological Process: regulation of kinase activity; inhibition of NF-kappaB transcription factor; innate immune response; positive regulation of MHC class I biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; defense response to virus; negative regulation of interferon type I production