Rabbit anti-Human, mouse NOD1 Polyclonal Antibody | anti-NOD1 antibody

NOD1 Antibody (C-term)

Cat. Num. AAA9210162

• Gene Names: NOD1; CARD4; NLRC1; CLR7.1
• Reactivity: Human, mouse
• Applications: Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NOD1; Polyclonal Antibody; NOD1 Antibody (C-term); Nucleotide-binding oligomerization domain-containing protein 1; Caspase recruitment domain-containing protein 4; CARD4; anti-NOD1 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-951 amino acids from the C-terminal region of human NOD1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 923-951
• Sequence Length: 953

• Applicable Applications for anti-NOD1 antibody: WB, E
• Application Notes: WB~~1:1000
• Function: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection.
• Cellular Location: Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Note=Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells
• Tissue Location: Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Testing Data (TD)

( All lanes : Anti-NOD1 Antibody (C-term) at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: NCI-H1299 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: SW480 whole cell lysate Lane 6: Mouse heart lysate Lane 7: Mouse spleen lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST)

Testing Data (TD)

( All lanes : Anti-NOD1 Antibody (C-term) at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: NCI-H1299 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: SW480 whole cell lysate Lane 6: Mouse heart lysate Lane 7: Mouse spleen lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NOD1 antibody

This gene encodes a member of the NOD (nucleotide-binding
oligomerization domain) family. This member is a cytosolic protein.
It contains an N-terminal caspase recruitment domain (CARD), a
centrally located nucleotide-binding domain (NBD), and 10 tandem
leucine-rich repeats (LRRs) in its C terminus. The CARD is involved
in apoptotic signaling, LRRs participate in protein-protein
interactions, and mutations in the NBD may affect the process of
oligomerization and subsequent function of the LRR domain. This
protein is an intracellular pattern-recognition receptor (PRR) that
initiates inflammation in response to a subset of bacteria through
the detection of bacterial diaminopimelic acid. Multiple
alternatively spliced transcript variants differring in the 5' UTR
have been described, but the full-length nature of these variants
has not been determined.

Product Categories/Family for anti-NOD1 antibody

Cell Biology

References

Hutton, M.L., et al. Infect. Immun. 78(11):4523-4531(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Lu, W.G., et al. World J. Gastroenterol. 16(34):4348-4356(2010)
Enevold, C., et al. Mult. Scler. 16(8):942-949(2010)
Ashton, K.A., et al. BMC Cancer 10, 382 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 5174617
• NCBI GeneID: 10392
• NCBI Accession #: NP_006083.1
• NCBI GenBank Nucleotide #: NM_006092.2
• UniProt Accession #: Q9Y239; Q549U4; Q8IWF5; B4DTU3
• Molecular Weight: 107691
• NCBI Official Full Name: nucleotide-binding oligomerization domain-containing protein 1
• NCBI Official Synonym Full Names: nucleotide-binding oligomerization domain containing 1
• NCBI Official Symbol: NOD1
• NCBI Official Synonym Symbols: CARD4; NLRC1; CLR7.1
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 1
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 1
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: NOD1
• UniProt Synonym Gene Names: CARD4
• UniProt Entry Name: NOD1_HUMAN

NCBI Description

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Oct 2009]

Uniprot Description

NOD1: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: basolateral plasma membrane; apical plasma membrane; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; peptidoglycan binding; protein homodimerization activity; CARD domain binding; caspase activator activity; ATP binding

Biological Process: detection of bacterium; apoptosis; activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; stress-activated MAPK cascade; positive regulation of caspase activity; positive regulation of JNK cascade; defense response; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; interleukin-8 biosynthetic process; JNK cascade; detection of biotic stimulus; inflammatory response; toll-like receptor 4 signaling pathway; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway