Rabbit anti-Human, Mouse NNT1 Polyclonal Antibody | anti-CLCF1 antibody

NNT1 Antibody

Cat. Num. AAA9629262

• Gene Names: CLCF1; CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NNT1; Polyclonal Antibody; NNT1 Antibody; B cell stimulating factor 3; B-cell-stimulating factor 3; BSF 3; BSF-3; BSF3; Cardiotrophin like cytokine; Cardiotrophin like cytokine factor 1; Cardiotrophin-like cytokine factor 1; CISS 2; CISS2; CLC; CLCF 1; Clcf1; CLCF1_HUMAN; Cold induced sweating syndrome 2; CRLF 1 associated cytokine like factor 1; CRLF1 associated cytokine like factor 1; Neurotrophin 1; Neurotrophin 1/B cell stimulating factor 3; Neurotrophin1; NNT 1; NNT-1; Novel neurotrophin 1; Novel neurotrophin-1; NR 6; NR6; anti-CLCF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: NNT1 Antibody detects endogenous levels of total NNT1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CLCF1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the IL-6 superfamily.
• Subunit Structure: Forms a heteromeric complex with cardiotrophin-like cytokine CRLF1/CLF-1; the CRLF1-CLCF1 complex is a ligand for the ciliary neurotrophic factor receptor/CNTFR. The CRLF1-CLCF1 heterodimer binds SORL1 (via N-terminal ectodomain); within this complex, the interaction is mediated predominantly by the CRLF1 moiety. The tripartite signaling complex formed by CRLF1, CLCF1 and CNTFR also binds SORL1.
• Tissue Specificity: Expressed predominantly in lymph nodes, spleen, peripheral blood lymphocytes, bone marrow, and fetal liver.
• Function: In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CLCF1 antibody

Secreted; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 7019351
• NCBI GeneID: 23529
• NCBI Accession #: NP_037378.1
• NCBI GenBank Nucleotide #: NM_013246.2
• UniProt Accession #: Q9UBD9; Q6NZA4; B4DNT4
• Molecular Weight: 25,176 Da
• NCBI Official Full Name: cardiotrophin-like cytokine factor 1 isoform 1
• NCBI Official Synonym Full Names: cardiotrophin-like cytokine factor 1
• NCBI Official Symbol: CLCF1
• NCBI Official Synonym Symbols: CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1
• NCBI Protein Information: cardiotrophin-like cytokine factor 1; novel neurotrophin-1; B-cell stimulating factor 3; B-cell stimulatory factor 3; B-cell-stimulating factor 3; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3
• UniProt Protein Name: Cardiotrophin-like cytokine factor 1
• Protein Family: Cardiotrophin-like cytokine factor
• UniProt Gene Name: CLCF1
• UniProt Synonym Gene Names: BSF3; CLC; NNT1; BSF-3; NNT-1
• UniProt Entry Name: CLCF1_HUMAN

NCBI Description

This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]

Uniprot Description

CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; protein heterodimerization activity; cytokine activity; ciliary neurotrophic factor receptor binding; receptor binding

Biological Process: positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; B cell differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; negative regulation of neuron apoptosis; JAK-STAT cascade; positive regulation of isotype switching to IgE isotypes; positive regulation of astrocyte differentiation; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Cold-induced Sweating Syndrome 2