Rabbit nNOS Polyclonal Antibody | anti-nNOS antibody

nNOS antibody

Cat. Num. AAA536053

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: To be determined by end-user
• Applications: Western Blot
• Purity: nNOS antibody was purified by affinity chromatography.
• Synonyms: nNOS; Polyclonal Antibody; nNOS antibody; Polyclonal nNOS; Anti-nNOS; Neuronal Nitric Oxide Synthase; anti-nNOS antibody

• Host: Rabbit
• Reactivity: To be determined by end-user
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: nNOS antibody was purified by affinity chromatography.
• Form/Format: Affinity purified IgG supplied in PBS with 0.02% NaN3.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1554

• Applicable Applications for anti-nNOS antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1,000
• Biological Significance: Human nitric oxide synthases are a family of enzymes responsible for the synthesis of nitric oxide from L-arginine and molecular oxygen. There are at least three nitric oxide synthases; NOS I, also known as neuronal NOS or nNOS, NOS II, which is referred to as inducible NOS or iNOS and NOS III, also known as endothelial NOS or eNOS. nNOS plays a role in the upregulation of microglial phospho-p38 MAPK. Such p38 MAPK activation in microglia is consistent with a potential role in the development of morphine antinociceptive tolerance.
• Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
• Immunogen: nNOS antibody was raised in rabbit using residues 19-31 [PRKGPPLPNGDTEC] of NOS as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-nNOS antibody

Affinity purified Rabbit polyclonal nNOS antibody

Product Categories/Family for anti-nNOS antibody

Proteases; Inhibitors; & Enzymes; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 642518
• NCBI GeneID: 4842
• UniProt Accession #: O75713; E9PH30; P29475
• Molecular Weight: 164,779 Da
• NCBI Official Full Name: neuronal nitric oxide synthase
• NCBI Official Synonym Full Names: nitric oxide synthase 1 (neuronal)
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: cytoskeleton; protein complex; photoreceptor inner segment; sarcoplasmic reticulum; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft

Molecular Function: calmodulin binding; protein binding; sodium channel regulator activity; FAD binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; FMN binding; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding

Biological Process: interaction with host; negative regulation of serotonin uptake; negative regulation of calcium ion transport; striated muscle contraction; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; peptidyl-cysteine S-nitrosylation; response to hypoxia; arginine catabolic process; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of blood pressure; blood coagulation; nitric oxide biosynthetic process; multicellular organismal response to stress; nitric oxide mediated signal transduction; myoblast fusion; neurotransmitter biosynthetic process

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1