Rabbit anti-Human NNMT Polyclonal Antibody | anti-NNMT antibody

NNMT, ID (NNMT, Nicotinamide N-methyltransferase) (Azide free) (HRP)

Cat. Num. AAA6325899

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NNMT; Polyclonal Antibody; ID (NNMT; Nicotinamide N-methyltransferase) (Azide free) (HRP); Nicotinamide N-methyltransferase; anti-NNMT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NNMT antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-130 amino acids from the Central region of human NNMT.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NNMT Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NNMT antibody detected the NNMT protein (arrow).)

Immunohistochemistry (IHC)

(NNMT Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NNMT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NNMT antibody

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq].

Product Categories/Family for anti-NNMT antibody

Antibodies; Enzymes; Methyltransferase

References

Giusti, B., et al. Thromb. Haemost. 104(2):231-242(2010). Zhang, J., et al. J Zhejiang Univ Sci B 11(2):136-143(2010). Emanuelli, M., et al. Histol. Histopathol. 25(1):15-20(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): van Driel, L.M., et al. J. Nutr. 139(12):2315-2321(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5453790
• NCBI GeneID: 4837
• NCBI Accession #: NP_006160.1
• NCBI GenBank Nucleotide #: NM_006169.2
• UniProt Accession #: P40261
• Molecular Weight: 29,574 Da
• NCBI Official Full Name: nicotinamide N-methyltransferase
• NCBI Official Synonym Full Names: nicotinamide N-methyltransferase
• NCBI Official Symbol: NNMT
• NCBI Protein Information: nicotinamide N-methyltransferase
• UniProt Protein Name: Nicotinamide N-methyltransferase
• UniProt Gene Name: NNMT
• UniProt Entry Name: NNMT_HUMAN

NCBI Description

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

Uniprot Description

NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.1.1.1; Methyltransferase

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function: nicotinamide N-methyltransferase activity

Biological Process: response to drug; methylation; organ regeneration; xenobiotic metabolic process; response to organic nitrogen