Rabbit Nna1 Polyclonal Antibody

Nna1 (Nervous system nuclear protein induced by axotomy 1, AGTPBP1, Cytosolic carboxypeptidase-1)

Cat. Num. AAA619079

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Nna1; Polyclonal Antibody; Nna1 (Nervous system nuclear protein induced by axotomy 1; AGTPBP1; Cytosolic carboxypeptidase-1); Anti -Nna1 (Nervous system nuclear protein induced by axotomy 1; anti-Nna1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recongnizes endog-enous levels of human total Nna1 protein. Species crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-Nna1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,; 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Asp1175 of human Nna1 protein (KLH-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from fetal and adult rat brain using MBS619079.)

Related Product Information for anti-Nna1 antibody

Nervous system nuclear protein induced by axotomy 1 (Nna1, AGTPBP1 or cytosolic carboxypeptidase-1) is related to zinc carboxypeptidases and contains an ATP/GTP binding motif, a basic and a bipartite nuclear localization signal. Nna1 expression is rapidly induced

Product Categories/Family for anti-Nna1 antibody

Antibodies; Abs to Enzymes; Carboxypeptidase

NCBI and Uniprot Product Information

• NCBI GI #: 11528062
• NCBI Official Full Name: nuclear ATP/GTP-binding protein

Uniprot Description

Nna1: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5. Interacts with MYLK. Belongs to the peptidase M14 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.17.-

Cellular Component: mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: peptidase activity; tubulin binding; protein binding; zinc ion binding; metallopeptidase activity; hydrolase activity; carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity

Biological Process: mitochondrion organization and biogenesis; neurotransmitter metabolic process; retina development in camera-type eye; olfactory bulb development; neuromuscular process; cerebellar Purkinje cell differentiation; cerebellar Purkinje cell layer development; cerebellum development; eye photoreceptor cell differentiation; proteolysis; adult walking behavior