Sequence Information
Kallikrein 1 (KLK1) Recombinant Protein | KLK1 recombinant protein
Recombinant Kallikrein 1 (KLK1)
Gene Names
Klk1; Ngfg; rK-1; rGK-1; Klk1b3; Klk1c1
Reactivity
Rat
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
Purity
>98%
Synonyms
Kallikrein 1 (KLK1); Recombinant Kallikrein 1 (KLK1); KLK1 recombinant protein
Host
E. coli
Reactivity
Rat
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Concentration
200ug/mL (varies by lot)
Applicable Applications for KLK1 recombinant protein
SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Val21~Pro261
Tags
N-terminal His-Tag
Tissue Specificity
Spleen, Kidney
Traits
Freeze-dried powder
Predicted isoelectric point
4.9
Predicted Molecular Mass
27.8kDa
Accurate Molecular Mass
31kDa as determined by SDS-PAGE reducing conditions
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
kallikrein-1
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
Klk1
NCBI Official Synonym Symbols
Ngfg; rK-1; rGK-1; Klk1b3; Klk1c1
NCBI Protein Information
kallikrein-1; gamma-NGF; PS kallikrein; pancreatic kallikrein; true tissue kallikrein; nerve growth factor gamma chain; kallikrein 1-related peptidase b3; nerve growth factor gamma polypeptide
UniProt Protein Name
Kallikrein-1
Protein Family
UniProt Gene Name
Ngfg
UniProt Synonym Gene Names
Klk-1; Klk1; rK-1
UniProt Entry Name
KLK1_RAT
NCBI Description
a putative pancreatic kallikrein [RGD, Feb 2006]
Uniprot Description
Catalytic activity: Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Tissue specificity: High levels in pancreas, submaxillary and parotid glands, spleen, and kidney. Ref.1
Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.Contains 1 peptidase S1 domain.
Sequence caution: The sequence AAA41464.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH78784.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA00346.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on KLK1
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Product Notes
The KLK1 ngfg (Catalog #AAA2012313) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Kallikrein 1 (KLK1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kallikrein 1 (KLK1) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the KLK1 ngfg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kallikrein 1 (KLK1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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