Rabbit NMDAR NR2B Polyclonal Antibody | anti-NMDA antibody

RABBIT ANTI NMDA RECEPTOR NR2B (pTyr1252)

Cat. Num. AAA221522

• Gene Names: Grin2b; GluN2B
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence)
• Applications: Western Blot
• Synonyms: NMDAR NR2B; Polyclonal Antibody; RABBIT ANTI NMDA RECEPTOR NR2B (pTyr1252); anti-NMDA antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor when phosphorylated at Tyrosine 1252. Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease (Popke, E.J.) and also epilepsy and ischemic neuronal cell death.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 1482

• Applicable Applications for anti-NMDA antibody: Western Blot (WB)*
• Application Notes: Western Blot: This item detects a band/s of approximately 180kDa in rat hippocampal cell lysates.; Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 50% Glycerol; 0.01% Bovine Serum Albumin
• Immunogen: Phosphopeptide corresponding to smino acid residues surrounding the phosphorylated tyrosine 1252 of the NR2B subunit of rat NMDA receptor.; Buffer Solution
• Antiserum Preparation: Antisera to rat NMDAR NR2B subunit pTyr1252 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-NMDA antibody

MBS221522 specifically recognises the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor when phosphorylated at Tyrosine 1252. Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease (Popke, E.J.) and also epilepsy and ischemic neuronal cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 6980984
• NCBI GeneID: 24410
• NCBI Accession #: NP_036706.1
• NCBI GenBank Nucleotide #: NM_012574.1
• UniProt Accession #: Q00960; Q62684
• Molecular Weight: 166,071 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2B
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• NCBI Official Symbol: Grin2b
• NCBI Official Synonym Symbols: GluN2B
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2B; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B; glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor, ionotropic, NMDA2B
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2B
• UniProt Gene Name: Grin2b
• UniProt Synonym Gene Names: GluN2B; NMDAR2B; NR2B
• UniProt Entry Name: NMDE2_RAT

NCBI Description

subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

Uniprot Description

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral; Channel, calcium

Cellular Component: neuron projection; cell surface; postsynaptic density; dendritic spine; terminal button; Z disc; N-methyl-D-aspartate selective glutamate receptor complex; presynaptic membrane; postsynaptic membrane; synaptic vesicle; membrane; synapse; cell junction

Molecular Function: neurotransmitter binding; interleukin-1 receptor binding; zinc ion binding; calcium channel activity; beta-catenin binding; drug binding; ionotropic glutamate receptor binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding; cation channel activity; cell adhesion molecule binding; receptor binding; D2 dopamine receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; behavioral fear response; startle response; response to toxin; rhythmic process; positive regulation of glutamate secretion; regulation of synaptic plasticity; response to other organism; response to organic cyclic substance; receptor clustering; positive regulation of synaptic transmission; response to carbohydrate stimulus; synaptic transmission; response to magnesium ion; behavioral response to pain; learning and/or memory; calcium ion transport; response to methylmercury; response to electrical stimulus; cation transport; associative learning; cellular response to magnesium starvation; regulation of action potential; synaptic transmission, glutamatergic; in utero embryonic development; hippocampus development; response to amphetamine; learning; response to cocaine; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; regulation of postsynaptic membrane potential; response to ethanol; sensory organ development; long-term memory; suckling behavior; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; regulation of MAPKKK cascade; response to cytokine stimulus; cerebral cortex development; regulation of excitatory postsynaptic membrane potential; response to calcium ion; multicellular organismal response to stress; response to amine stimulus