Rabbit LSP (pS190) Antibody | anti-LSP (pS190) antibody

Rabbit anti LSP1, Phosphospecific

Cat. Num. AAA462231

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Synonyms: LSP (pS190); Rabbit anti LSP1; Phosphospecific; Rabbit anti-phosphor-LSP1; Lymphocyte antigen; lymphocyte-specific protein 1 (LSP1);; anti-LSP (pS190) antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Specificity: This antibody recognizes ~52 kDa of human LSP1 protein at a phosphorylated serine 252. It does not recognize non-phosphorylated peptides confirmed by dot blot. This antibody also reacts with mouse. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-LSP (pS190) antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide corresponding to the C-terminus with a phosphorylated serine of human lymphocyte-specific protein 1. This sequence is identical to mouse species
• Positive Control: Mouse spleen tissue
• Cellular Location: Cytoplasmic and membrane
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-LSP (pS190) antibody

Leukocyte-specific protein 1 (LSP1), an F-actin binding protein and a major downstream substrate of p38 mitogen-activated protein kinase as well as protein kinase C, is important in leukocyte chemotaxis. Two serine residues at positions 204 and 252 are potential phosphorylation sites. The amino acid sequences surrounding these two sites are in agreement with the consensus sequence (Xaa-Xaa-Hyd-Xaa-Arg-Xaa-Xaa-Ser-Xaa-Xaa) for phosphorylation by MAPKAP kinase 2. Both serine residues in human LSP1 and the corresponding conserved serine residues in human and mouse LSP1 are in the basic Cterminal F-actin binding domain. LSP1 is a substrate for MAPKAP kinase 2 in vitro and that the phosphorylation sites are located in the basic C-terminal domain of LSP1.

References

Lixin Liu, et al. LSP1 is an endothelial gatekeeper of leukocyte transendothelial migration. (2005), JEM, 201 (3): 409-418.

NCBI and Uniprot Product Information

• NCBI GI #: 581127
• UniProt Accession #: P00804
• Molecular Weight: ~52 kDa
• NCBI Official Full Name: lsp
• UniProt Protein Name: Lipoprotein signal peptidase
• UniProt Gene Name: lspA
• UniProt Entry Name: LSPA_ECOLI

Uniprot Description

Function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins. HAMAP MF_00161

Catalytic activity: Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161

Subcellular location: Cell inner membrane; Multi-pass membrane protein HAMAP MF_00161.

Sequence similarities: Belongs to the peptidase A8 family.