Rabbit anti-Human NKX3-2 Polyclonal Antibody | anti-NKX3-2 antibody

NKX3-2, ID (NKX3-2, BAPX1, NKX3B, Homeobox protein Nkx-3.2, Bagpipe homeobox protein homolog 1, Homeobox protein NK-3 homolog B) (Azide free) (HRP)

Cat. Num. AAA6325635

• Gene Names: NKX3-2; SMMD; BAPX1; NKX3B; NKX3.2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NKX3-2; Polyclonal Antibody; ID (NKX3-2; BAPX1; NKX3B; Homeobox protein Nkx-3.2; Bagpipe homeobox protein homolog 1; Homeobox protein NK-3 homolog B) (Azide free) (HRP); Homeobox protein NK-3 homolog B; anti-NKX3-2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NKX3-2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NKX3-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-235 amino acids from the Central region of human NKX3-2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NKX3-2 Antibody (Center) western blot analysis in U266 cell line lysates (35ug/lane).This demonstrates the NKX3-2 antibody detected the NKX3-2 protein (arrow).)

Related Product Information for anti-NKX3-2 antibody

This gene encodes a member of the NK family of homeobox-containing proteins. The encoded protein may play a role in skeletal development.

Product Categories/Family for anti-NKX3-2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Hellemans, J., et al. Am. J. Hum. Genet. 85(6):916-922(2009). Rodrigo, I., et al. Mol. Cell. Biol. 24(7):2757-2766(2004). Tribioli, C., et al. Gene 203(2):225-233(1997). Yoshiura, K.I., et al. Genomics 45(2):425-428(1997). Tribioli, C., et al. Mech. Dev. 65 (1-2), 145-162 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4502365
• NCBI GeneID: 579
• NCBI Accession #: NP_001180.1
• NCBI GenBank Nucleotide #: NM_001189.3
• UniProt Accession #: P78367; Q2M2I7
• Molecular Weight: 34,814 Da
• NCBI Official Full Name: homeobox protein Nkx-3.2
• NCBI Official Synonym Full Names: NK3 homeobox 2
• NCBI Official Symbol: NKX3-2
• NCBI Official Synonym Symbols: SMMD; BAPX1; NKX3B; NKX3.2
• NCBI Protein Information: homeobox protein Nkx-3.2; homeobox protein NK-3 homolog B; bagpipe homeobox protein homolog 1
• UniProt Protein Name: Homeobox protein Nkx-3.2
• Protein Family: Homeobox protein
• UniProt Gene Name: NKX3-2
• UniProt Synonym Gene Names: BAPX1; NKX3B
• UniProt Entry Name: NKX32_HUMAN

NCBI Description

This gene encodes a member of the NK family of homeobox-containing proteins. The encoded protein may play a role in skeletal development. [provided by RefSeq, Jul 2008]

Uniprot Description

NKX3-2: Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus. Defects in NKX3-2 are the cause of spondylo- megaepiphyseal-metaphyseal dysplasia (SMMD). It is a skeletal dysplasia characterized by disproportionate short stature with a short and stiff neck and trunk, relatively long limbs that may show flexion contractures of the distal joints, delayed and impaired ossification of the vertebral bodies, the presence of large epiphysea ossification centers and wide growth plates in the long tubular bones and numerous pseudoepiphyses of the short tubular bones in hands and feet. Belongs to the NK-3 homeobox family.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleus

Biological Process: transcription from RNA polymerase II promoter; spleen development; organ formation; skeletal morphogenesis; embryonic skeletal development; negative regulation of chondrocyte differentiation; pancreas development; middle ear morphogenesis; negative regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; skeletal development; negative regulation of apoptosis

Disease: Spondylo-megaepiphyseal-metaphyseal Dysplasia