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Immunohistochemistry (IHC) (Anti-Nkx2-4 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Rabbit anti-Human NKX2-4 Polyclonal Antibody | anti-NKX2-4 antibody

Rabbit Polyclonal to Human NKX2-4

Gene Names
Nkx2-4; tinman; Nkx-2.4; 1700001P03Rik
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
NKX2-4; Polyclonal Antibody; Rabbit Polyclonal to Human NKX2-4; Anti-NKX2-4 Antibody (Internal) IHC-plus; Homeobox protein Nkx-2.4; NKX2.4; NKX2D; NK-2 (Drosophila) homolog D; NK-2 homolog D; NK2 homeobox 4; Nkx-2.4; NKX24; Tinman; Human NKX2-4; anti-NKX2-4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human NKX24. NKX2-4 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-4 are known to exist; this antibody will detect both isoforms.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
354
Applicable Applications for anti-NKX2-4 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1-2 ug/mL.
Target Species
Human
Immunogen Description
An 18 amino acid peptide near the center of human NKX2-4.
Immunogen Type
Synthetic peptide
Immunogen
NKX2-4 antibody was raised against an 18 amino acid peptide near the center of human NKX2-4.
Antigen Modification
Internal
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-Nkx2-4 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-Nkx2-4 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of NKX2-4 in A20 cells with NKX2-4 antibody at 20 ug/ml.)

Immunofluorescence (IF) (Immunofluorescence of NKX2-4 in A20 cells with NKX2-4 antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of NKX2-4 in A20 cells with NKX2-4 antibody at 2.5 ug/ml.)

Immunocytochemistry (ICC) (Immunocytochemistry of NKX2-4 in A20 cells with NKX2-4 antibody at 2.5 ug/ml.)

Western Blot (WB)

(Western blot analysis of NKX2-4 in A20 cell lysate with NKX2-4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

Western Blot (WB) (Western blot analysis of NKX2-4 in A20 cell lysate with NKX2-4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,225 Da
NCBI Official Full Name
homeobox protein Nkx-2.4
NCBI Official Synonym Full Names
NK2 homeobox 4
NCBI Official Symbol
Nkx2-4
NCBI Official Synonym Symbols
tinman; Nkx-2.4; 1700001P03Rik
NCBI Protein Information
homeobox protein Nkx-2.4; homeobox protein NK-2 homolog D; NK2 transcription factor related, locus 4
UniProt Protein Name
Homeobox protein Nkx-2.4
Protein Family
UniProt Gene Name
Nkx2-4
UniProt Synonym Gene Names
Nkx2d
UniProt Entry Name
NKX24_MOUSE

Uniprot Description

NKX2-4: Probable transcription factor. Belongs to the NK-2 homeobox family.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding

Biological Process: regulation of transcription, DNA-dependent; multicellular organismal development

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Product Notes

The NKX2-4 nkx2-4 (Catalog #AAA248006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human NKX2-4 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NKX2-4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (5 ug/ml), WB (1 - 2 ug/ml) Usage: NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1-2 ug/mL. Researchers should empirically determine the suitability of the NKX2-4 nkx2-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKX2-4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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