Rabbit anti-Human, Mouse NKIRAS1 Polyclonal Antibody | anti-NKIRAS1 antibody

NKIRAS1 Antibody

Cat. Num. AAA9609384

• Gene Names: NKIRAS1; KBRAS1; kappaB-Ras1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: NKIRAS1; Polyclonal Antibody; NKIRAS1 Antibody; 2400004O09Rik; I kappa B interacting Ras like protein 1; I-kappa-B-interacting Ras-like protein 1; Kappa B Ras 1; Kappa B Ras protein 1; Kappa B-Ras protein 1; KappaB Ras1; KappaB-Ras1; KBRAS 1; KBRAS1; KBRS1_HUMAN; NF kappa B inhibitor interacting Ras like protein 1; NF-kappa-B inhibitor-interacting Ras-like protein 1; NFKB inhibitor interacting Ras like 1; NFKB inhibitor interacting Ras like protein 1; NKIRAS 1; nkiras1; anti-NKIRAS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NKIRAS1 antibody detects endogenous levels of total NKIRAS1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 192

• Applicable Applications for anti-NKIRAS1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:200
• Immunogen: A synthesized peptide derived from human NKIRAS1
• Subcellular Location: Cytoplasm.
• Tissue Specificity: Widely expressed.
• Predicted Cross Reactivity: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (83%), Horse (100%), Sheep (92%), Rabbit (92%), Dog (100%), Chicken (83%), Xenopus (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9609384 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis NKIRAS1 using mouse liver lysates)

Related Product Information for anti-NKIRAS1 antibody

Function: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP-and GDP-bound forms block phosphorylation of NFKBIB.
Subunit Structure: Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo. Forms a complex with NFKBIB and NF-kappa-B heterodimer (p50/NFKB1 and p65/RELA). Also interacts with c-Rel (REL).
Similarity: In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Leu residues, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 9966809
• NCBI GeneID: 28512
• NCBI Accession #: NP_065078.1
• NCBI GenBank Nucleotide #: NM_020345.3
• UniProt Accession #: Q9NYS0; Q96K18
• Molecular Weight: Observed: 22 kDa; Predicted: 22 kDa
• NCBI Official Full Name: NF-kappa-B inhibitor-interacting Ras-like protein 1
• NCBI Official Synonym Full Names: NFKB inhibitor interacting Ras like 1
• NCBI Official Symbol: NKIRAS1
• NCBI Official Synonym Symbols: KBRAS1; kappaB-Ras1
• NCBI Protein Information: NF-kappa-B inhibitor-interacting Ras-like protein 1
• UniProt Protein Name: NF-kappa-B inhibitor-interacting Ras-like protein 1
• Protein Family: NF-kappa-B inhibitor-interacting Ras-like protein
• UniProt Gene Name: NKIRAS1
• UniProt Synonym Gene Names: KBRAS1; Kappa B-Ras protein 1; KappaB-Ras1

Uniprot Description

Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.