Rabbit anti-Rat NKCC2 Polyclonal Antibody
NKCC2 (Sodium, Potassium, Chloride Cotransporter 2, BSC1, CCC2)
Serum
Serum
Dilution: Western Blot: 1:1000-1:5000. Full length huma NKCC2 is ~150kD.
ELISA: 1:10,000-1:50,000. Using 1ug/ml control peptide per well.
Unlike NKCC1, NKCC2 (human 1099 aa, rat/mouse 1095 aa; calculated mol size of ~121kD; actual size ~150kD; ~45% identity with NKCC1) is most strongly expressed in the kidney (inner and outer stripes of medulla and cortical thick ascending limb), and macula densa. NKCC2 plays a critical role in transcellular absorption of Na+Cl- by the medullary and cortical TALH, and a secondary role in the paracellular transport of Na-Ca and Mg. Mutations in the NKCC2 gene result in Bartter's syndrome, an inherited disease characterized by hypokalemic metabolic alkalosis, hypercalciuria, salt wasting, and volume depletion.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010]
Uniprot Description
NKCC2: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Kidney specific. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 15q15-q21.1
Cellular Component: membrane; apical plasma membrane; plasma membrane; integral to membrane
Molecular Function: sodium:potassium:chloride symporter activity
Biological Process: transport; sodium ion transport; ion transport; chloride transport; kidney development; excretion; transmembrane transport; potassium ion transport; chemical homeostasis
Disease: Bartter Syndrome, Antenatal, Type 1
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Product Notes
The NKCC2 (Catalog #AAA615157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NKCC2 (Sodium, Potassium, Chloride Cotransporter 2, BSC1, CCC2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NKCC2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000-1:5000. Full length huma NKCC2 is ~150kD. ELISA: 1:10,000-1:50,000. Using 1ug/ml control peptide per well. Researchers should empirically determine the suitability of the NKCC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKCC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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