Human Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit | NOSIP elisa kit

Human Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit

Cat. Num. AAA454444

• Gene Names: NOSIP; CGI-25
• Reactivity: Human
• Synonyms: Nitric Oxide Synthase Interacting Protein (NOSIP); Human Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit; NOSIP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NOSIP. No significant cross-reactivity or interference between NOSIP and analogues was observed.
• Sequence Length: 301

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NOSIP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NOSIP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOSIP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NOSIP in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOSIP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NOSIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NOSIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NOSIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 401709927
• NCBI GeneID: 51070
• NCBI Accession #: NP_001257889.1
• NCBI GenBank Nucleotide #: NM_001270960.1
• UniProt Accession #: Q9Y314; Q96FD2
• Molecular Weight: 33,172 Da
• NCBI Official Full Name: nitric oxide synthase-interacting protein
• NCBI Official Synonym Full Names: nitric oxide synthase interacting protein
• NCBI Official Symbol: NOSIP
• NCBI Official Synonym Symbols: CGI-25
• NCBI Protein Information: nitric oxide synthase-interacting protein
• UniProt Protein Name: Nitric oxide synthase-interacting protein
• Protein Family: Nitric oxide synthase-interacting protein
• UniProt Gene Name: NOSIP

NCBI Description

The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012]

Uniprot Description

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).