Rabbit anti-Human NIK Polyclonal Antibody | anti-MAP4K4 antibody

NIK, CT (NF-kappa-beta-inducing Kinase, HsNIK, FTDCR1B, HS, Mitogen-activated Protein Kinase Kinase Kinase 14, MAP3K14, Serine/threonine Protein Kinase NIK)

Cat. Num. AAA6000261

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified; Purified IgG
• Synonyms: NIK; Polyclonal Antibody; CT (NF-kappa-beta-inducing Kinase; HsNIK; FTDCR1B; HS; Mitogen-activated Protein Kinase Kinase Kinase 14; MAP3K14; Serine/threonine Protein Kinase NIK); Anti -NIK; anti-MAP4K4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NIK.
• Purity/Purification: Purified; Purified IgG
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-MAP4K4 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Peptide corresponding to amino acids 931-947 of human NIK (1).
• Positive Control: 293 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of CARD9 expression in human HL-60 cell lysate with anti-CARD9 at 1 ug /ml.)

Related Product Information for anti-MAP4K4 antibody

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, LPS and mitogens. A serine/threonine protein kinase which mediates NF-kB activation by IL-1, TNFa and CD95 was identified recently and designated NIK (for NF-kB inducing kinase) (1). NIK is an activator of IkB kinase alpha and beta (IKKa and IKKb) (2-5). Therefore, NIK is a key molecule in the NF-kB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (1,2). Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells (3,4). CCR5 is required for the infection by HIV-1, HIV-2, and SIV (1,2). The b-chemokines RANTES, MIP-1a and MIP-1b are the ligands for CCR5 and prevent infection by M-tropic HIV-1 (3-5). CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells (6,7). The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites (8,9). CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

Product Categories/Family for anti-MAP4K4 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 3721838
• NCBI GeneID: 9448
• UniProt Accession #: O95819; O75172; Q9NST7
• Molecular Weight: 142,101 Da
• NCBI Official Full Name: NIK
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; Ste20 group protein kinase HGK; MAPK/ERK kinase kinase kinase 4; hepatocyte progenitor kinase-like/germinal center kinase-like kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• Protein Family: Nik-related protein kinase
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
• UniProt Entry Name: M4K4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.1; STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 2q11.2-q12

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: microvillus biogenesis; regulation of catalytic activity; regulation of JNK cascade; protein amino acid phosphorylation