Rabbit anti-Human MAP4K4 / NIK Polyclonal Antibody | anti-MAP4K4 antibody

Rabbit Polyclonal to Human MAP4K4 / NIK

Cat. Num. AAA240974

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: MAP4K4 / NIK; Polyclonal Antibody; Rabbit Polyclonal to Human MAP4K4 / NIK; Anti-MAP4K4 / NIK Antibody (aa931-947) IHC-plus; MAP4K4; HGK; HPK/GCK-like kinase HGK; KIAA0687; Misshapen; NIK; Nck-interacting kinase; HPK/GCK-like kinase; Ste20 group protein kinase HGK; MEKKK 4; MEKKK4; Zc1; FLH21957; MEK kinase kinase 4; Human MAP4K4; anti-MAP4K4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1320

• Applicable Applications for anti-MAP4K4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (20 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Peptide corresponding to amino acids 931 to 947 of human NIK.
• Immunogen: MAP4K4 / NIK antibody was raised against peptide corresponding to amino acids 931 to 947 of human NIK.
• Antigen Modification: aa931-947
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MAP4K4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Related Product Information for anti-MAP4K4 antibody

MAP4K4 is a MSN protein kinase. The protein has a structure similar to other GCK (Group I) kinases and contains two consensus SH3 binding motifs, which have been shown to interact with the SH3 domains of the SH2/SH3 adapter protein Nck. NIK can be activated by tumor necrosis factor (TNF)-alpha. Activated NIK has been shown to activate c-Jun N-terminal kinase (JNK) and to bind and activate the plasma membrane Na-H exchanger NHE1.

Product Categories/Family for anti-MAP4K4 antibody

Family: Protein Kinase; Subfamily: MSN

NCBI and Uniprot Product Information

• NCBI GI #: 22035604
• NCBI GeneID: 9448
• NCBI Accession #: NP_663719.1
• NCBI GenBank Nucleotide #: NM_145686.2
• UniProt Accession #: O95819; O75172; Q9NST7
• Molecular Weight: 141,929 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 4 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; Ste20 group protein kinase HGK; MAPK/ERK kinase kinase kinase 4; epididymis secretory protein Li 31; hepatocyte progenitor kinase-like/germinal center kinase-like kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
• UniProt Entry Name: M4K4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 2q11.2-q12

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: microvillus biogenesis; regulation of catalytic activity; protein amino acid phosphorylation; regulation of JNK cascade