Rabbit NID2 Polyclonal Antibody | anti-NID2 antibody

NID2 Polyclonal Antibody

Cat. Num. AAA3007049

• Gene Names: NID2; NID-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NID2; Polyclonal Antibody; NID2 Polyclonal Antibody; Nidogen-2; NID-2; Osteonidogen; anti-NID2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NID2 polyclonal antibody detects endogenous levels of NID2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1375

• Applicable Applications for anti-NID2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human NID2
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NID2 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-NID2 antibody

Nidogens are highly conserved proteins present in vertebrate and invertebrate basement membranes. Nidogens connect the Laminin and Collagen IV networks and integrate other proteins into the membrane. In mammals, two Nidogen proteins, Nidogen and Nidogen-2, interact at comparable levels with Collagen I, IV and Perlecan, serving to stabilize basement membranes and playing a major role in embryogenesis. The two isoforms have a similar shape, consisting of three globular domains, and co-localize in vessel walls and other basement membrane zones. Nidogen-2 is a cell adhesion protein glycosylated at nitrogen and oxygen sites, and is widely distributed in basement membranes in heart, placenta, bone and, to a lesser extent, in pancreas, kidney and skeletal muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 144953895
• NCBI GeneID: 22795
• NCBI Accession #: NP_031387.3
• NCBI GenBank Nucleotide #: NP_031387.3
• UniProt Accession #: Q14112
• Molecular Weight: ~ 151kDa
• NCBI Official Full Name: nidogen-2
• NCBI Official Synonym Full Names: nidogen 2
• NCBI Official Symbol: NID2
• NCBI Official Synonym Symbols: NID-2
• NCBI Protein Information: nidogen-2
• UniProt Protein Name: Nidogen-2
• Protein Family: Nidogen
• UniProt Gene Name: NID2
• UniProt Synonym Gene Names: NID-2

NCBI Description

This gene encodes a member of the nidogen family of basement membrane proteins. This protein is a cell-adhesion protein that binds collagens I and IV and laminin and may be involved in maintaining the structure of the basement membrane.[provided by RefSeq, Jun 2010]

Uniprot Description

Nidogen-2: a cell adhesion glycoprotein widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q22.1

Cellular Component: basement membrane; extracellular exosome; extracellular matrix; extracellular region; plasma membrane

Molecular Function: calcium ion binding; collagen binding; protein binding

Biological Process: basement membrane organization; cell adhesion; cell-matrix adhesion; extracellular matrix organization