anti-Human NHE5 Polyclonal Antibody | anti-NHE5 antibody

Anti - NHE5 (Na, H Exchanger 5)

Cat. Num. AAA613738

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity purified over the control-peptide Sepharose
• Synonyms: NHE5; Polyclonal Antibody; Anti - NHE5 (Na; H Exchanger 5); NHE5 (Na; anti-NHE5 antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NHE5. No significant homology exists with other NHE isoforms (NHE1-4).
• Purity/Purification: Affinity purified over the control-peptide Sepharose
• Form/Format: Affinity Purified; Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NHE5 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA and Western Blot. Other applications not tested.; Dilution: ELISA: 1:100,000 using 50-100ng control peptide/well; Western Blot: 1-10ug/ml using Chemiluminescence; Optimal dilutions to be determined by the researcher.
• Immunogen: A 13aa peptide within the cytoplasmic domain of rat NHE5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NHE5 antibody

Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, and regression of cancer and renal intestinal disorders. Five NHE isoforms (NHE1-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.

Product Categories/Family for anti-NHE5 antibody

Abs to Ion Channel

References

• 1. Attaphitya S et al (1999) J Biol. Chem. 274, 4383-4388.; Baird NR et al (1999) J Biol. Chem. 274, 4377-4382; Klanke CA et al (1995) Genomics 25, 615-622; Chris Yun CH et al (1995) Am J Physiol. 269, G1-G11 (Review); Josette N and Pouyssegur J (1995) Am J Physiol. 268, C283-C296 (review).