Rabbit anti-Human NGB Polyclonal Antibody | anti-NGB antibody

NGB, NT (NGB, Neuroglobin) (PE)

Cat. Num. AAA6325179

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NGB; Polyclonal Antibody; NT (NGB; Neuroglobin) (PE); Neuroglobin; anti-NGB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NGB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NGB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NGB.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NGB Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the NGB antibody detected the NGB protein (arrow).)

Immunohistochemistry (IHC)

(NGB Antibody (N-term) (AP14753a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NGB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NGB antibody

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions.

Product Categories/Family for anti-NGB antibody

Antibodies; Proteins

References

Li, R.C., et al. J. Cereb. Blood Flow Metab. 30(11):1874-1882(2010). Astudillo, L., et al. Biophys. J. 99 (2), L16-L18 (2010): Chuang, P.Y., et al. J. Neurotrauma 27(6):999-1006(2010). Mu, J., et al. Spectrochim Acta A Mol Biomol Spectrosc 75(5):1600-1604(2010). Raychaudhuri, S., et al. Apoptosis 15(4):401-411(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 10864065
• NCBI GeneID: 58157
• NCBI Accession #: NP_067080.1
• NCBI GenBank Nucleotide #: NM_021257.3
• UniProt Accession #: Q9NPG2
• Molecular Weight: 16,933 Da
• NCBI Official Full Name: neuroglobin
• NCBI Official Synonym Full Names: neuroglobin
• NCBI Official Symbol: NGB
• NCBI Protein Information: neuroglobin
• UniProt Protein Name: Neuroglobin
• Protein Family: Neuroglobin
• UniProt Gene Name: NGB
• UniProt Entry Name: NGB_HUMAN

NCBI Description

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008]

Uniprot Description

NGB: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. Belongs to the globin family.

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: mitochondrion; perikaryon

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: oxygen transport; apoptosis