Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with NFKBp65 Antibody(C-termS536). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFKBp65 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NFKBp65 immunoreactivity is localized to Cytoplasm significantly.)
Rabbit NFKBp65 Polyclonal Antibody | anti-RELA antibody
NFKBp65 Antibody (C-term S536)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with NFKBp65 Antibody(C-termS536). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFKBp65 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NFKBp65 immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with NFKBp65 Antibody(C-termS536). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFKBp65 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NFKBp65 immunoreactivity is localized to Cytoplasm significantly.)
Western Blot (WB)
(NFKBp65 Antibody (pS536) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow).)
Western Blot (WB)
(NFKBp65 Antibody (pS536) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow).)
(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex.
The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of
NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA,
MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by
trapping it in the cytoplasm. Phosphorylation of serine residues on
the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM
603258) marks them for destruction via the ubiquitination pathway,
thereby allowing activation of the NFKB complex. Activated NFKB
complex translocates into the nucleus and binds DNA at
kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or
5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G
purine; and Y is a C or T pyrimidine).
Tago, K., et al. J. Biol. Chem. 285(40):30622-30633(2010)
Park, J.S., et al. Oncol. Rep. 24(3):709-714(2010)
Yu, Z.H., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):650-652(2010)
Rohwer, N., et al. PLoS ONE 5 (8), E12038 (2010) :
NCBI and Uniprot Product Information
NCBI Description
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.
Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity
Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; hair follicle development; toll-like receptor 4 signaling pathway; defense response to virus; response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; membrane protein intracellular domain proteolysis; acetaldehyde metabolic process; response to amino acid stimulus; toll-like receptor 2 signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; response to mechanical stimulus; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of protein catabolic process; response to progesterone stimulus; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; transcription from RNA polymerase II promoter; response to cAMP; response to morphine; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; response to insulin stimulus; response to organic substance; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; aging; positive regulation of Schwann cell differentiation; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; liver development; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-12 biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; response to cobalamin
Research Articles on RELA
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Product Notes
The RELA rela (Catalog #AAA9200856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 517-539. The NFKBp65 Antibody (C-term S536) reacts with Human (Predicted Reactivity: Pig, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's NFKBp65 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFKBp65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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