Western Blot (WB)
(Western Blot of NFKB p65 antibody. Lane 1-4: PC3 cell lysate. Load: 20, 30, 40, and 50 ug per lane. NFKB p65 antibody at 1:5000 for overnight at 4 deg C. HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: ~65kDa for NFKB p65. Other band(s): ~110kDa and ~45kDa.)
Rabbit NFKB P65 Rel A Polyclonal Antibody | anti-NFKB P65 Rel A antibody
NFKB P65 Rel A antibody
Reactivity
Human NFKB p65
Applications
ELISA, Western Blot
Synonyms
NFKB P65 Rel A; Polyclonal Antibody; NFKB P65 Rel A antibody; Polyclonal NFKB P65 Rel A; Anti-NFKB P65 Rel A; Nuclear factor NF-kappa-B p65 subunit; nfkb; NF-kappaB; NFkappaB; NF-kB; NFKB; anti-NFKB P65 Rel A antibody
Host
Rabbit
Reactivity
Human NFKB p65
Clonality
Polyclonal
Specificity
Human NFKB p65
Form/Format
Delipidation and defibrination rabbit anti-serum, sterile filtered with 0.01% sodium azide.
Applicable Applications for anti-NFKB P65 Rel A antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:5,000-1:25,000
WB: 1:500-1:3,000
WB: 1:500-1:3,000
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
NFKB P65 rel A antibody was raised in rabbit using NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH as the immunogen.
Preparation and Storage
Store at -20 degree C. Aliquot and freeze at -20 degree C or below for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot of NFKB p65 antibody. Lane 1-4: PC3 cell lysate. Load: 20, 30, 40, and 50 ug per lane. NFKB p65 antibody at 1:5000 for overnight at 4 deg C. HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: ~65kDa for NFKB p65. Other band(s): ~110kDa and ~45kDa.)
Western Blot (WB)
(Western Blot of NFKB p65 antibody. Lane 1-4: PC3 cell lysate. Load: 20, 30, 40, and 50 ug per lane. NFKB p65 antibody at 1:5000 for overnight at 4 deg C. HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: ~65kDa for NFKB p65. Other band(s): ~110kDa and ~45kDa.)
Related Product Information for anti-NFKB P65 Rel A antibody
Rabbit polyclonal NFKB P65 Rel A antibody
Product Categories/Family for anti-NFKB P65 Rel A antibody
Similar Products
Product Notes
The NFKB P65 Rel A (Catalog #AAA534196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFKB P65 Rel An antibody reacts with Human NFKB p65 and may cross-react with other species as described in the data sheet. AAA Biotech's NFKB P65 Rel A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:5,000-1:25,000 WB: 1:500-1:3,000. Researchers should empirically determine the suitability of the NFKB P65 Rel A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFKB P65 Rel A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
