Western Blot (WB)
(Staining (0.5ug/ml) of HepG2 lysate (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat NFIL3 Polyclonal Antibody | anti-NFIL3 antibody
Goat anti-NFIL3 Antibody
Western Blot: Approx.55-60kDa band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al. , Proc Natl Acad Sci U S A.1997 Mar 18;94(6): 2609-14.; PMID: 9122243). Recommended concentration: 0.5-1.5ug/ml.
Western Blot (WB)
(Staining (0.5ug/ml) of HepG2 lysate (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.5ug/ml) of HepG2 lysate (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
Uniprot Description
E4BP4: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B- site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Belongs to the bZIP family. NFIL3 subfamily.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 9q22
Cellular Component: nucleus
Molecular Function: DNA binding; protein binding; transcription corepressor activity; transcription factor activity
Biological Process: circadian rhythm; immune response; negative regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Research Articles on NFIL3
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Product Notes
The NFIL3 nfil3 (Catalog #AAA420749) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NFIL3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NFIL3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx.55-60kDa band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A.1997 Mar 18;94(6): 2609-14.; PMID: 9122243). Recommended concentration: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the NFIL3 nfil3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RLIATQPISA SDSG. It is sometimes possible for the material contained within the vial of "NFIL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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