Rabbit anti-Human NFATC4 Polyclonal Antibody | anti-NFATC4 antibody

NFATC4 (Nuclear Factor of Activated T-cells, Cytoplasmic 4, NF-ATc4, NFATc4, T-cell Transcription Factor NFAT3, NF-AT3, NFAT3)

Cat. Num. AAA649270

• Gene Names: NFATC4; NFAT3; NF-ATc4
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NFATC4; Polyclonal Antibody; NFATC4 (Nuclear Factor of Activated T-cells; Cytoplasmic 4; NF-ATc4; NFATc4; T-cell Transcription Factor NFAT3; NF-AT3; NFAT3); Anti -NFATC4 (Nuclear Factor of Activated T-cells; anti-NFATC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NFATC4.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MGAASCEDEELEFKLVFGEEKEAPPLGAGGLGEELDSEDAPPCCRLALGEPPPYGAAPIGIPRPPPPRPGMHSPPPRPAPSPGTWESQPARSVRLGGPGGGAGGAGGGRVLECPSIRITSISPTPEPPAALEDNPDAWGDGSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFGLGSPLPSPRASPRPWTPEDPWSLYGPSPGGRGPEDSWLLLSAPGPTPASPRPASPCGKRRYSSSGTPSSASPALSRRGSLGEEGSEPPPPPPLPLARDPGSPGPFDYVGAPPAESIPQKTRRTSSEQAVALPRSEEPASCNGKLPLGAEESVAPPGGSRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELRIEVQPRAHHRAHYETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERNLRPHAFYQVHRITGKMVATASYEAVVSGTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQGGGKVVSVQAASVPIECSQRSAQELPQVEAYSPSACSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQWEEEATVNRLQSNEVTLTLTVPEYSNKRVSRPVQVYFYVSNGRRKRSPTQSFRFLPVICKEEPLPDSSLRGFPSASATPFGTDMDFSPPRPPYPSYPHEDPACETPYLSEGFGYGMPPLYPQTGPPPSYRPGLRMFPETRGTTGCAQPPAVSFLPRPFPSDPYGGRGSSFSLGLPFSPPAPFRPPPLPASPPLEGPFPSQSDVHPLPAEGYNKVGPGYGPGEGAPEQEKSRGGYSSGFRDSVPIQGITLEEVSEIIGRDLSGFPAPPGEEPPA

• Applicable Applications for anti-NFATC4 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human NFATC4, aa1-902 (NP_004545.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NFATC4 expression in transfected 293T cell line by NFATC4 polyclonal antibody. Lane 1: NFATC4 transfected lysate (95.4kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis of protein-protein interactions between NFATC4 and MAPK14 HeLa cells were stained with NFATC4 rabbit purified polyclonal 1:1200 and MAPK14 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Related Product Information for anti-NFATC4 antibody

The protein is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4.

Product Categories/Family for anti-NFATC4 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 27886563
• NCBI GeneID: 4776
• NCBI Accession #: NP_004545.2
• NCBI GenBank Nucleotide #: NM_004554.4
• UniProt Accession #: Q14934; B4DDG5; B4DY55; B5B2U7; B5B2U8; B5B2U9; B5B2V0; B5B2V1; B5B2V2; B5B2V3; B5B2V4; B5B2V5
• Molecular Weight: 95,449 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 4 isoform 2
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
• NCBI Official Symbol: NFATC4
• NCBI Official Synonym Symbols: NFAT3; NF-ATc4
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 4; NF-AT3; T cell transcription factor NFAT3; T-cell transcription factor NFAT3
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 4
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: NFATC4
• UniProt Synonym Gene Names: NFAT3; NF-ATc4; NFATc4; NF-AT3
• UniProt Entry Name: NFAC4_HUMAN

NCBI Description

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

Function: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons. Ref.1 Ref.7 Ref.9 Ref.10

Subunit structure: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. Interacts with CREBBP, GATA4, IRAK1, MAPK8, MAPK9 and RPS6KA3. Ref.6 Ref.8 Ref.9 Ref.14

Subcellular location: Cytoplasm. Nucleus. Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. Ref.13

Tissue specificity: Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus. Ref.2

Domain: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors

By similarity.

Post-translational modification: Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B. Ref.7 Ref.8 Ref.9 Ref.11 Ref.13Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'. Ref.11

Sequence similarities: Contains 1 IPT/TIG domain.Contains 1 RHD (Rel-like) domain.