Western Blot (WB)
(NFATC4 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NFATC4 antibody detected the NFATC4 protein (arrow).)
Rabbit anti-Human NFATC4 Polyclonal Antibody | anti-NFATC4 antibody
NFATC4, CT (NFATC4, NFAT3, Nuclear factor of activated T-cells, cytoplasmic 4, T-cell transcription factor NFAT3) (AP)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(NFATC4 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NFATC4 antibody detected the NFATC4 protein (arrow).)
Western Blot (WB)
(NFATC4 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NFATC4 antibody detected the NFATC4 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of U251 cell stained with NFATC4 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFATC4 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFATC4 immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of U251 cell stained with NFATC4 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFATC4 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFATC4 immunoreactivity is localized to Cytoplasm significantly.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
NFAT3: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. 23 alternatively spliced isoforms of the human protein hve been reported. Note: This description may include information from UniProtKB
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: transcription factor complex; intermediate filament cytoskeleton; cytoplasm; nucleus; cytosol
Molecular Function: peroxisome proliferator activated receptor binding; protein binding; transcription coactivator activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; patterning of blood vessels; DNA damage response, signal transduction resulting in induction of apoptosis; heart development; cellular respiration; muscle cell development; regulation of synaptic plasticity; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; positive regulation of tumor necrosis factor production
Research Articles on NFATC4
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Product Notes
The NFATC4 nfatc4 (Catalog #AAA6324949) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFATC4, CT (NFATC4, NFAT3, Nuclear factor of activated T-cells, cytoplasmic 4, T-cell transcription factor NFAT3) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFATC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NFATC4 nfatc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFATC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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