Rabbit anti-Human, Mouse NFAT4 Polyclonal Antibody | anti-NFAT4 antibody

NFAT4 (Ser165) Antibody

Cat. Num. AAA9412913

• Gene Names: NFATC3; NFAT4; NFATX
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: NFAT4; Polyclonal Antibody; NFAT4 (Ser165) Antibody; NFATX; ; anti-NFAT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of NFAT4 only when phosphorylated at Serine 165.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1068

• Applicable Applications for anti-NFAT4 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 165
• Modification: Phospho-Ser165
• Target Name: NFAT4
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from Hela cells using NFAT4 (Phospho-Ser165) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-NFAT4 antibody

Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.

Product Categories/Family for anti-NFAT4 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4758804
• NCBI GeneID: 4775
• NCBI Accession #: NP_004546.1
• NCBI GenBank Nucleotide #: NM_004555.3
• UniProt Accession #: Q12968; O75211; Q14516; Q99840; Q99841; Q99842
• Molecular Weight: 80,582 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 3 isoform 2
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• NCBI Official Symbol: NFATC3
• NCBI Official Synonym Symbols: NFAT4; NFATX
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 3
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 3
• UniProt Gene Name: NFATC3
• UniProt Synonym Gene Names: NFAT4; NF-ATc3; NFATc3; NF-AT4
• UniProt Entry Name: NFAC3_HUMAN

NCBI Description

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

NFAT4: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells. Plays a role in the regulation of gene expression in T cells and immature thymocytes. Six spliced isoforms have been identified. Isoform 1 is predominantly expressed in thymus, peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; transcription from RNA polymerase II promoter; heart development; cellular respiration; muscle cell development; innate immune response; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response