Rabbit NFAT3 Polyclonal Antibody | anti-NFAT3 antibody

NFAT3 Antibody

Cat. Num. AAA9611177

• Gene Names: NFATC4; NFAT3; NF-AT3; NF-ATC4
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NFAT3; Polyclonal Antibody; NFAT3 Antibody; cytoplasmic 4; NF ATc4; NF-AT3; NF-ATc4; NFAC4_HUMAN; NFATc4; Nuclear factor of activated T cells cytoplasmic 4; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4; Nuclear factor of activated T-cells; T cell transcription factor NFAT3; T-cell transcription factor NFAT3; anti-NFAT3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NFAT3 Antibody detects endogenous levels of NFAT3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 964

• Applicable Applications for anti-NFAT3 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human NFAT3.
• Tissue Specificity: Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of NFAT3 in lysates of HepG2 , using NFAT3 Antibody.)

Related Product Information for anti-NFAT3 antibody

Description: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.

Post Translational Modifications: Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B. Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'.

Subunit Structure: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. Interacts with CREBBP, GATA4, IRAK1, MAPK8, MAPK9 and RPS6KA3.

Similarity: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.

NCBI and Uniprot Product Information

• NCBI GI #: 209862845
• NCBI GeneID: 4776
• NCBI Accession #: NP_001129494.1
• NCBI GenBank Nucleotide #: NP_001129494.1
• UniProt Accession #: Q14934
• Molecular Weight: Observed: 95 kDa; Predicted: 96 kDa
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 4 isoform 1
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 4
• NCBI Official Symbol: NFATC4
• NCBI Official Synonym Symbols: NFAT3; NF-AT3; NF-ATC4
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 4
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 4
• UniProt Gene Name: NFATC4
• UniProt Synonym Gene Names: NFAT3; NF-ATc4; NFATc4; NF-AT3
• UniProt Entry Name: NFAC4_HUMAN

NCBI Description

This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

NFAT3: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. 23 alternatively spliced isoforms of the human protein hve been reported. Note: This description may include information from UniProtKB

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: transcription factor complex; intermediate filament cytoskeleton; cytoplasm; nucleus; cytosol

Molecular Function: peroxisome proliferator activated receptor binding; protein binding; transcription coactivator activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; patterning of blood vessels; DNA damage response, signal transduction resulting in induction of apoptosis; heart development; cellular respiration; muscle cell development; regulation of synaptic plasticity; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; positive regulation of tumor necrosis factor production