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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human ovary tumor using MBS768874 (EEF1A2 antibody) at dilution of 1:100)

Rabbit EEF1A2 Polyclonal Antibody | anti-EEF1A2 antibody

anti- EEF1A2 antibody

Gene Names
EEF1A2; HS1; STN; EF1A; STNL; MRD38; EEF1AL; EIEE33; EF-1-alpha-2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
>=95% as determined by SDS-PAGE
Purification: Immunogen Affinity Purified
Synonyms
EEF1A2; Polyclonal Antibody; anti- EEF1A2 antibody; eEF1A 2; EEF1AL; EF 1 alpha 2; EF1A; Elongation factor 1 alpha 2; FLJ41696; HS1; Statin S1; STN; STNL; anti-EEF1A2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, Mouse, Rat; other species are not tested. Please decide the specificity by homology
Purity/Purification
>=95% as determined by SDS-PAGE
Purification: Immunogen Affinity Purified
Form/Format
Liquid
Sequence Length
463
Applicable Applications for anti-EEF1A2 antibody
ELISA, WB, IHC, IP
Application Notes
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
Preparation and Storage
-20 degree C for 24 months (Avoid repeated freeze/thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human ovary tumor using MBS768874 (EEF1A2 antibody) at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human ovary tumor using MBS768874 (EEF1A2 antibody) at dilution of 1:100)

Immunoprecipitation (IP)

(IP Result of anti-EEF1A2 (IP: MBS768874, 4ug; Detection: MBS768874 1:1000) with mouse brain tissue lysate 4000ug.)

Immunoprecipitation (IP) (IP Result of anti-EEF1A2 (IP: MBS768874, 4ug; Detection: MBS768874 1:1000) with mouse brain tissue lysate 4000ug.)

Western Blot (WB)

(MCF7 cells were subjected to SDS PAGE followed by western blot with MBS768874 (EEF1A2 antibody) at dilution of 1:500)

Western Blot (WB) (MCF7 cells were subjected to SDS PAGE followed by western blot with MBS768874 (EEF1A2 antibody) at dilution of 1:500)
Related Product Information for anti-EEF1A2 antibody
Background: EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50-53kd
NCBI Official Full Name
elongation factor 1-alpha 2
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 2
NCBI Official Symbol
EEF1A2
NCBI Official Synonym Symbols
HS1; STN; EF1A; STNL; MRD38; EEF1AL; EIEE33; EF-1-alpha-2
NCBI Protein Information
elongation factor 1-alpha 2
UniProt Protein Name
Elongation factor 1-alpha 2
UniProt Gene Name
EEF1A2
UniProt Synonym Gene Names
EEF1AL; STN; EF-1-alpha-2; eEF1A-2
UniProt Entry Name
EF1A2_HUMAN

NCBI Description

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014]

Uniprot Description

EEF1A2: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: cell soma; cytoplasm; eukaryotic translation elongation factor 1 complex; myelin sheath; nucleus

Molecular Function: GTP binding; GTPase activity; protein binding; protein kinase binding; translation elongation factor activity; translation factor activity, nucleic acid binding

Biological Process: positive regulation of apoptosis; response to electrical stimulus; response to inorganic substance; translational elongation

Disease: Epileptic Encephalopathy, Early Infantile, 33; Mental Retardation, Autosomal Dominant 38

Research Articles on EEF1A2

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Product Notes

The EEF1A2 eef1a2 (Catalog #AAA768874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The anti- EEF1A2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1A2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB, IHC, IP. WB : 1:500-1:5000 IP : 1:500-1:5000 IHC : 1:20-1:200. Researchers should empirically determine the suitability of the EEF1A2 eef1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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