Rabbit NF-YA Polyclonal Antibody | anti-NF-YA antibody

NF-YA Polyclonal Antibody

Cat. Num. AAA9405862

• Gene Names: NFYA; HAP2; CBF-A; CBF-B; NF-YA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: NF-YA; Polyclonal Antibody; NF-YA Polyclonal Antibody; NFYA; Nuclear transcription factor Y subunit alpha; CAAT box DNA-binding protein subunit A; Nuclear transcription factor Y subunit A; anti-NF-YA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: NF-YA Polyclonal Antibody detects endogenous levels of NF-YA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 347

• Applicable Applications for anti-NF-YA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: Western Blot: 1:500 - 1:2000; Immunohistochemistry: 1:100 - 1:300; Immunofluorescence: 1:200 - 1:1000; ELISA: 1:20000; Not yet tested in other applications.
• Immunogen Type: peptide
• Immunogen Description: Synthesized peptide derived from the C-terminal region of human NF-YA.
• Target Name: NF-YA
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of COLO205 cells using NF-YA Polyclonal Antibody)

Product Categories/Family for anti-NF-YA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4505389
• NCBI GeneID: 4800
• NCBI Accession #: NP_002496.1
• NCBI GenBank Nucleotide #: NM_002505.4
• UniProt Accession #: P23511; Q8IXU0
• Molecular Weight: 33,939 Da
• NCBI Official Full Name: nuclear transcription factor Y subunit alpha isoform 1
• NCBI Official Synonym Full Names: nuclear transcription factor Y, alpha
• NCBI Official Symbol: NFYA
• NCBI Official Synonym Symbols: HAP2; CBF-A; CBF-B; NF-YA
• NCBI Protein Information: nuclear transcription factor Y subunit alpha
• UniProt Protein Name: Nuclear transcription factor Y subunit alpha
• UniProt Gene Name: NFYA
• UniProt Synonym Gene Names: NF-YA
• UniProt Entry Name: NFYA_HUMAN

NCBI Description

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

NFYA: stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. Involved in cisplatin resistance. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; rhythmic process; positive regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process