Immunofluorescence (IF)
(Immunofluorescent analysis of a section of adult mouse cerebellar dentate nucleus stained with chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:3,000 in red. The section was costained with rabbit pAb to GFAP dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA.)
Chicken Neuron Specific Enolase (NSE) Polyclonal Antibody | anti-NSE antibody
Neuron Specific Enolase (NSE)
Antibody is supplied as an aliquot of serum.
IF: 1:500
IHC: 1:500
WB: 1:2,000
Immunofluorescence (IF)
(Immunofluorescent analysis of a section of adult mouse cerebellar dentate nucleus stained with chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:3,000 in red. The section was costained with rabbit pAb to GFAP dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA.)
Immunofluorescence (IF)
(Immunofluorescent analysis of a section of adult mouse cerebellar dentate nucleus stained with chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:3,000 in red. The section was costained with rabbit pAb to GFAP dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA.)
Western Blot (WB)
(Western blot analysis of tissue and cell lysates using chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow brain, and [5] SH-SY5Y cells. the strong band running at about 47kDa corresponds to the NSE protein.)
Western Blot (WB)
(Western blot analysis of tissue and cell lysates using chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow brain, and [5] SH-SY5Y cells. the strong band running at about 47kDa corresponds to the NSE protein.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Uniprot Description
ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: extracellular space; photoreceptor inner segment; phosphopyruvate hydratase complex; plasma membrane; perikaryon; cytosol
Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity
Biological Process: glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis
Research Articles on NSE
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Product Notes
The NSE eno2 (Catalog #AAA555976) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Neuron Specific Enolase (NSE) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuron Specific Enolase (NSE) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:500 IF: 1:500 IHC: 1:500 WB: 1:2,000. Researchers should empirically determine the suitability of the NSE eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuron Specific Enolase (NSE), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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