Goat anti-Mouse, Rat Neuroglycan C Polyclonal Antibody | anti-CSPG5 antibody
Neuroglycan C (Chondroitin 5 Sulfate Proteoglycan Acidic Leucine-rich EGF-like Domain-containing Brain Protein, CSPG5)
Gene Names
CSPG5; NGC; MGC44034; CSPG5
Reactivity
Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by mouse Neuroglycan C affinity chromatography.
Synonyms
Neuroglycan C; Polyclonal Antibody; Neuroglycan C (Chondroitin 5 Sulfate Proteoglycan Acidic Leucine-rich EGF-like Domain-containing Brain Protein; CSPG5); Anti -Neuroglycan C (Chondroitin 5 Sulfate Proteoglycan Acidic Leucine-rich EGF-like Domain-containing Brain Protein; anti-CSPG5 antibody
Host
Goat
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Neuroglycan C, extracellular domain. In direct ELISA, this antibody shows 25% cross-reactivity with rhNeuroglycan C. Species Crossreactivity: Mouse, rat.
Purity/Purification
Affinity Purified
Purified by mouse Neuroglycan C affinity chromatography.
Purified by mouse Neuroglycan C affinity chromatography.
Form/Format
Lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.
Applicable Applications for anti-CSPG5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for Immunohistochemistry ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 10ug/ml detects Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked
with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT After blocking, cells were incubated with diluted primary antibody for 3 hours at RTfollowed by donkey anti-goat lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 10ug/ml detects Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked
with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT After blocking, cells were incubated with diluted primary antibody for 3 hours at RTfollowed by donkey anti-goat lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Immunogen
purified, CHO cell-derived, recombinant mouse Neuroglycan C (rmNeuroglycan C; aa 31-420; Accession# AAH55736).
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-CSPG5 antibody
Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-160kD member of the neuregulin family of proteins. Depending upon its expression profile, it may be a glycoprotein oft 120kD, or a chondroitin sulfate (CS) proteoglycan of 140-160kD. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons. Mature human NGC is a 536 amino acid (aa) type I transmembrane glycoprotein. It possesses a 393 aa extracellular region (aa 31-423) that contains a Ser117 CS attachment site and an EGF-like domain aa 371-413). There are at least two isoform variants. One shows a deletion of aa87-513, while a second shows an alternative start site at Met139 with the same deletion. Phosphorylation likely occurs on Ser249, and proteolysis generates a > 75kD soluble fragment. Over aa 31-420, human NGC shares 84% aa identity with mouse NGC.
Product Categories/Family for anti-CSPG5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,016 Da
NCBI Official Full Name
neuroglycan C
NCBI Official Synonym Full Names
chondroitin sulfate proteoglycan 5 (neuroglycan C)
NCBI Official Symbol
CSPG5
NCBI Official Synonym Symbols
NGC; MGC44034; CSPG5
NCBI Protein Information
chondroitin sulfate proteoglycan 5; neuroglycan C; OTTHUMP00000210429; acidic leucine-rich EGF-like domain-containing brain protein
UniProt Protein Name
Chondroitin sulfate proteoglycan 5
Protein Family
UniProt Gene Name
CSPG5
UniProt Synonym Gene Names
CALEB; NGC
UniProt Entry Name
CSPG5_HUMAN
NCBI Description
The protein encoded by this gene is a proteoglycan that may function as a neural growth and differentiation factor. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
Uniprot Description
CSPG5: May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: Golgi apparatus; lysosomal lumen; endoplasmic reticulum membrane; membrane; integral to plasma membrane; Golgi lumen; extracellular region; integral to membrane
Molecular Function: protein binding; growth factor activity
Biological Process: chondroitin sulfate metabolic process; regulation of synaptic transmission; nervous system development; chondroitin sulfate biosynthetic process; intracellular transport; glycosaminoglycan metabolic process; regulation of growth; axon regeneration; carbohydrate metabolic process; chondroitin sulfate catabolic process; pathogenesis; dermatan sulfate biosynthetic process
Research Articles on CSPG5
1. results suggest that NGC may be a novel candidate gene, and neuregulin signaling pathways may play an important role in schizophrenia
2. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
3. NGC IV, which was first found in the present study, had the shortest cytoplasmic domain among the four variants
4. neuroglycan C is a novel component of midkine receptors involved in process elongation
2. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
3. NGC IV, which was first found in the present study, had the shortest cytoplasmic domain among the four variants
4. neuroglycan C is a novel component of midkine receptors involved in process elongation
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Product Notes
The CSPG5 cspg5 (Catalog #AAA619988) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Neuroglycan C (Chondroitin 5 Sulfate Proteoglycan Acidic Leucine-rich EGF-like Domain-containing Brain Protein, CSPG5) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuroglycan C can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Suitable for Immunohistochemistry ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Immunohistochemistry: 10ug/ml detects Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT After blocking, cells were incubated with diluted primary antibody for 3 hours at RTfollowed by donkey anti-goat lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Researchers should empirically determine the suitability of the CSPG5 cspg5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuroglycan C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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