Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Antigen KI-67 (MKI67) Standard Curve.)
Mouse Antigen KI-67 (MKI67) ELISA Kit | MKI67 elisa kit
Mouse Antigen KI-67 (MKI67) ELISA Kit
Reactivity
Mouse
Synonyms
Antigen KI-67 (MKI67); Mouse Antigen KI-67 (MKI67) ELISA Kit; MKI67; RP11-380J17.2; KIA; proliferation-related Ki-67 antigen; MKI67 elisa kit
Reactivity
Mouse
Specificity
Mouse Antigen KI-67 (MKI67) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MKI67. No significant cross-reactivity or interference between Mouse MKI67 and analogues was observed.
Sequence Length
3119
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Antigen KI-67 (MKI67) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Antigen KI-67 (MKI67) Standard Curve.)
Related Product Information for MKI67 elisa kit
Description: This Mouse Antigen KI-67 (MKI67) ELISA Kit employs a two-site sandwich ELISA to quantitate MKI67 in samples. An antibody specific for MKI67 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MKI67 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKI67 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKI67 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The Ki-67 protein is a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes.Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).Ki-67 is an excellent marker to determine the growth fraction of a given cell population.The fraction of Ki-67-positive tumor cells (the Ki-67 Labeling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate and the breast.
Background: The Ki-67 protein is a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes.Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).Ki-67 is an excellent marker to determine the growth fraction of a given cell population.The fraction of Ki-67-positive tumor cells (the Ki-67 Labeling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate and the breast.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
antigen KI-67
Protein Family
Similar Products
Product Notes
The Mouse MKI67 (Catalog #AAA9718176) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718176 ELISA Kit recognizes Mouse MKI67. It is sometimes possible for the material contained within the vial of "Antigen KI-67 (MKI67), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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