Immunohistochemistry (IHC)
(Paraffin-embedded mouse embryo E12.5+ tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of MBS632276. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity is expressed in embryonic brain in synaptic processes. Staining pattern apears to be cytoplasmic.)
Rabbit anti-Mouse Neurogenin 3 Polyclonal Antibody | anti-NEUROG3 antibody
Neurogenin 3 (Ngn3, Neurogenin Family Helix-loop-helix Transcription Factor)
Serum
Serum
Dilution: Western Blot: 1:1000 detected Neurogenin 3 on 10ug of mNgn3 transfected rabbit reticulocyte lysates.
Immunohistochemistry: 1:500 detected Neurogenin 3 in mouse embryo E12.5+ tissue.
Immunohistochemistry (IHC)
(Paraffin-embedded mouse embryo E12.5+ tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of MBS632276. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity is expressed in embryonic brain in synaptic processes. Staining pattern apears to be cytoplasmic.)
Immunohistochemistry (IHC)
(Paraffin-embedded mouse embryo E12.5+ tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of MBS632276. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity is expressed in embryonic brain in synaptic processes. Staining pattern apears to be cytoplasmic.)
Chemiluminescence
(mNgn3 transfected (lane 2) and wildtype (lane 1) rabbit reticulocyte lysates were resolved by electrophoresis, transferred to PVDF membranes and probed with a 1:1000 dilution of MBS632276. Proteins were visualized using a donkey anti-rabbit (HRP) and chemiluminescence detection system. Arrow indicates Neurogenin 3 (~32kD).)
Chemiluminescence
(mNgn3 transfected (lane 2) and wildtype (lane 1) rabbit reticulocyte lysates were resolved by electrophoresis, transferred to PVDF membranes and probed with a 1:1000 dilution of MBS632276. Proteins were visualized using a donkey anti-rabbit (HRP) and chemiluminescence detection system. Arrow indicates Neurogenin 3 (~32kD).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).
Uniprot Description
neurogenin 3: Acts as a transcriptional regulator. Together with NKX2- 2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Defects in NEUROG3 are the cause of diarrhea type 4 (DIAR4). DIAR4 is a characterized by severe, life- threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells.
Protein type: Transcription factor; Cell development/differentiation; DNA-binding
Chromosomal Location of Human Ortholog: 10q21.3
Cellular Component: cytoplasm; nucleus
Molecular Function: protein dimerization activity; chromatin DNA binding; transcription coactivator activity; double-stranded DNA binding
Biological Process: nervous system development; central nervous system development; transcription, DNA-dependent; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; endocrine pancreas development; peripheral nervous system development; epithelial cell differentiation; spinal cord development; forebrain development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; hindbrain development
Disease: Diarrhea 4, Malabsorptive, Congenital
Research Articles on NEUROG3
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Product Notes
The NEUROG3 neurog3 (Catalog #AAA632276) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neurogenin 3 (Ngn3, Neurogenin Family Helix-loop-helix Transcription Factor) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Neurogenin 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:1000 detected Neurogenin 3 on 10ug of mNgn3 transfected rabbit reticulocyte lysates. Immunohistochemistry: 1:500 detected Neurogenin 3 in mouse embryo E12.5+ tissue. Researchers should empirically determine the suitability of the NEUROG3 neurog3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurogenin 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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