Western Blot (WB)
(Western Blot: Sample: Human jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human CIP2A Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Human Cancerous Inhibitor Of PP2A Polyclonal Antibody | anti-CIP2A antibody
Polyclonal Antibody to Cancerous Inhibitor Of PP2A (CIP2A)
Gene Names
CIP2A; p90; KIAA1524
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Cancerous Inhibitor Of PP2A; Polyclonal Antibody; Polyclonal Antibody to Cancerous Inhibitor Of PP2A (CIP2A); anti-CIP2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CIP2A. It has beenselected for its ability to recognize CIP2A in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-N IHQTFQLIFN ILINGDGTLT RKYSVDLLMD LLKNPKIADY LTRYEHFSSC LHQVLGLLNG KDPDSSSKVL ELLLAFCSVT QLRHMLTQMM FEQSPPGSAT LGSHTKCLEP TVALLRWLSQ PLDGSENCSV LALELFKEIF EDVIDAANCS SADRFVTLLL PTILDQLQFT EQNLDEALTR KKCERIAKAI EVLLTLCGDD TLKMHIAKIL TTVKCTTLIE QQFTYGKIDL GFGTKVADSE LCKLAADVIL KTLDLINKLK PLVPGMEVSF YKILQDPRLI TPLAFALTSD NREQVQSGLR ILLEAAPLPD
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-N IHQTFQLIFN ILINGDGTLT RKYSVDLLMD LLKNPKIADY LTRYEHFSSC LHQVLGLLNG KDPDSSSKVL ELLLAFCSVT QLRHMLTQMM FEQSPPGSAT LGSHTKCLEP TVALLRWLSQ PLDGSENCSV LALELFKEIF EDVIDAANCS SADRFVTLLL PTILDQLQFT EQNLDEALTR KKCERIAKAI EVLLTLCGDD TLKMHIAKIL TTVKCTTLIE QQFTYGKIDL GFGTKVADSE LCKLAADVIL KTLDLINKLK PLVPGMEVSF YKILQDPRLI TPLAFALTSD NREQVQSGLR ILLEAAPLPD
Applicable Applications for anti-CIP2A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CIP2A (Asn230~Glu560)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant CIP2A (Asn230~Glu560) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2085277
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human CIP2A Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Human jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human CIP2A Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Testis tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Testis tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,579 Da
NCBI Official Full Name
protein CIP2A
NCBI Official Synonym Full Names
cell proliferation regulating inhibitor of protein phosphatase 2A
NCBI Official Symbol
CIP2A
NCBI Official Synonym Symbols
p90; KIAA1524
NCBI Protein Information
protein CIP2A
UniProt Protein Name
Protein CIP2A
UniProt Gene Name
KIAA1524
UniProt Synonym Gene Names
CIP2A
Uniprot Description
CIP2A: an oncoprotein that inhibits PP2A in human malignancies. Like the SV40 small t antigen, inhibits the phospho-serine/-threonine phosphatase PP2A. Promotes c-Myc protein stability by inhibiting the dephosphorylation of phospho-serine 62. Expressed at low levels in most of nonmalignant tissues but at high levels in human malignancies including head and neck squamous cell carcinoma (HNSCC), colon cancer, and liver cancer. Antibodies against CIP2A are present in sera from many patients with gastric or prostate cancer. Two alternatively spliced human isoforms have been described.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q13.13
Cellular Component: cytoplasm; cytosol; integral component of membrane; plasma membrane
Molecular Function: cadherin binding; protein binding
Biological Process: spermatogenesis
Research Articles on CIP2A
Similar Products
Product Notes
The CIP2A kiaa1524 (Catalog #AAA2027783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cancerous Inhibitor Of PP2A (CIP2A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cancerous Inhibitor Of PP2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CIP2A kiaa1524 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-N IHQTFQLIFN ILINGDGTLT RKYSVDLLMD LLKNPKIADY LTRYEHFSSC LHQVLGLLNG KDPDSSSKVL ELLLAFCSVT QLRHMLTQMM FEQSPPGSAT LGSHTKCLEP TVALLRWLSQ PLDGSENCSV LALELFKEIF EDVIDAANCS SADRFVTLLL PTILDQLQFT EQNLDEALTR KKCERIAKAI EVLLTLCGDD TLKMHIAKIL TTVKCTTLIE QQFTYGKIDL GFGTKVADSE LCKLAADVIL KTLDLINKLK PLVPGMEVSF YKILQDPRLI TPLAFALTSD NREQVQSGLR ILLEAAPLPD. It is sometimes possible for the material contained within the vial of "Cancerous Inhibitor Of PP2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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