Rabbit anti-Human Neurogenin 3 Polyclonal Antibody | anti-NEUROG3 antibody

Neurogenin 3, NT (NEUROG3, ATOH5, BHLHA7, NGN3, Neurogenin-3, Class A basic helix-loop-helix protein 7, Protein atonal homolog 5) (MaxLight 650)

Cat. Num. AAA6324891

• Gene Names: NEUROG3; ngn3; Atoh5; NGN-3; Math4B; bHLHa7
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Neurogenin 3; Polyclonal Antibody; NT (NEUROG3; ATOH5; BHLHA7; NGN3; Neurogenin-3; Class A basic helix-loop-helix protein 7; Protein atonal homolog 5) (MaxLight 650); NEUROG3; Protein atonal homolog 5; anti-NEUROG3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Neurogenin3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-NEUROG3 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 40-69 in the N-terminal region of human Neurogenin3.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(All lanes: MBS6011760 (1:2000) Lane 1: DU145 whole cell lysate Lane 2: LNCap whole cell lysate Lane 3: PANC-1 whole cell lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit. Predicted band size: 23kD)

Western Blot (WB)

(Western Blot analysis of 293 cell lysates (2ug/lane) usingMBS6011760. Lane 1: Nontransfected Lane 2: transiently transfected with the Neurogenin3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human large intestine tissue using MBS6011760 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6011760 for immunohistochemistry.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NCI-H460 usingMBS6011760, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-NEUROG3 antibody

Antibodies; Transcription Factors

References

1. Heremans, Y., et al., J. Cell Biol. 159(2):303-312 (2002). 2. Sommer, L., et al., Mol. Cell. Neurosci. 8(4):221-241 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 229462908
• NCBI GeneID: 50674
• UniProt Accession #: Q9Y4Z2; Q5VVI0; Q6DJX6; Q9BY24
• Molecular Weight: 214
• NCBI Official Full Name: Neurogenin-3
• NCBI Official Synonym Full Names: neurogenin 3
• NCBI Official Symbol: NEUROG3
• NCBI Official Synonym Symbols: ngn3; Atoh5; NGN-3; Math4B; bHLHa7
• NCBI Protein Information: neurogenin-3; protein atonal homolog 5; class A basic helix-loop-helix protein 7
• UniProt Protein Name: Neurogenin-3
• Protein Family: Neurogenin
• UniProt Gene Name: NEUROG3
• UniProt Synonym Gene Names: ATOH5; BHLHA7; NGN3; NGN-3; bHLHa7
• UniProt Entry Name: NGN3_HUMAN

NCBI Description

The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).[provided by RefSeq, May 2010]

Uniprot Description

neurogenin 3: Acts as a transcriptional regulator. Together with NKX2- 2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Defects in NEUROG3 are the cause of diarrhea type 4 (DIAR4). DIAR4 is a characterized by severe, life- threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; chromatin DNA binding; double-stranded DNA binding; transcription coactivator activity

Biological Process: nervous system development; central nervous system development; transcription, DNA-dependent; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; endocrine pancreas development; peripheral nervous system development; epithelial cell differentiation; spinal cord development; forebrain development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of neuron differentiation; hindbrain development

Disease: Diarrhea 4, Malabsorptive, Congenital