SDS-Page
Alpha-Fetoprotein (aFP) Recombinant Protein | aFP recombinant protein
Recombinant Alpha-Fetoprotein (aFP)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Alpha-Fetoprotein (aFP); Recombinant Alpha-Fetoprotein (aFP); aFP recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-AHIHEECC RGNVLECLQD AERVVSYVCS QQDTLSSKIA ECCKLPTTLE LGQCIIHAEN DDKPEGLSPN LNRFLGERDFNQLSSREKDL SMARFTYEYS RRHPKLAVPV ILRVAKGYQE LLEKCS
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-AHIHEECC RGNVLECLQD AERVVSYVCS QQDTLSSKIA ECCKLPTTLE LGQCIIHAEN DDKPEGLSPN LNRFLGERDFNQLSSREKDL SMARFTYEYS RRHPKLAVPV ILRVAKGYQE LLEKCS
Sequence Length
610
Applicable Applications for aFP recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Sus scrofa; Porcine (Pig)
Expression System
Prokaryotic expression
Residues
Ala263~Ser386 (Accession # Q8MJ76) with two N-terminal Tags, His-tag and GST-tag
Subcellular Location
Secreted
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46.1kDa
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Protein Information
alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
Protein Family
UniProt Gene Name
AFP
UniProt Entry Name
FETA_PIG
Uniprot Description
Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin
By similarity.
Subunit structure: Dimeric and trimeric forms have been found in addition to the monomeric form
By similarity.
Subcellular location: Secreted
By similarity.
Tissue specificity: Plasma. Synthesized by the fetal liver and yolk sac.
Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.
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Product Notes
The aFP afp (Catalog #AAA2009616) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alpha-Fetoprotein (aFP) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the aFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -AHIHEECC RGNVLECLQD AERVVSYVCS QQDTLSSKIA ECCKLPTTLE LGQCIIHAEN DDKPEGLSPN LNRFLGERDF NQLSSREKDL SMARFTYEYS RRHPKLAVPV ILRVAKGYQE LLEKCS. It is sometimes possible for the material contained within the vial of "Alpha-Fetoprotein (aFP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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