Western Blot (WB)
(Anti-NEUROG2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: SH-SY5Y Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:29 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Neurogenin-2 Polyclonal Antibody | anti-NEUROG2 antibody
Anti-Neurogenin-2 Antibody, Rabbit Polyclonal
ICC/IF: 1:200-1:1000
IP: 4-8uL/mg of lysate
Western Blot (WB)
(Anti-NEUROG2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: SH-SY5Y Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:29 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-NEUROG2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: SH-SY5Y Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:29 kDa (We are unsure as to the identity of these extra bands.))
Immunofluorescence (IF)
(Immunofluorescence staining of NEUROG2 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NEUROG2 polyclonal antibody (dilution ratio 1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of NEUROG2 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NEUROG2 polyclonal antibody (dilution ratio 1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)
Immunoprecipitation (IP)
(NEUROG was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-NEUROG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NEUROG rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa)
Immunoprecipitation (IP)
(NEUROG was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-NEUROG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NEUROG rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a neural-specific basic helix-loop-helix (bHLH) transcription factor that can specify a neuronal fate on ectodermal cells and is expressed in neural progenitor cells within the developing central and peripheral nervous systems. The protein product of this gene also plays a role in the differentiation and survival of midbrain dopaminergic neurons. [provided by RefSeq, Apr 2012]
Uniprot Description
neurogenin 2: Transcriptional regulator. Involved in neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 4q25
Cellular Component: nucleus
Molecular Function: protein dimerization activity; sequence-specific DNA binding
Biological Process: axon guidance; cell fate commitment; transcription, DNA-dependent; forebrain development; cell maturation; neuron migration; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of neuron differentiation; central nervous system neuron development
Research Articles on NEUROG2
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Product Notes
The NEUROG2 neurog2 (Catalog #AAA8101128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Neurogenin-2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neurogenin-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ICC/IF: 1:200-1:1000 IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the NEUROG2 neurog2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurogenin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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