Rabbit anti-Human Neurogenin 1 Polyclonal Antibody | anti-NEUROG1 antibody

Neurogenin 1, NT (NEUROG1, BHLHA6, NEUROD3, NGN, NGN1, Neurogenin-1, Class A basic helix-loop-helix protein 6, Neurogenic basic-helix-loop-helix protein, Neurogenic differentiation factor 3) (Biotin)

Cat. Num. AAA6324852

• Gene Names: NEUROG1; AKA; ngn1; Math4C; bHLHa6; NEUROD3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Neurogenin 1; Polyclonal Antibody; NT (NEUROG1; BHLHA6; NEUROD3; NGN; NGN1; Neurogenin-1; Class A basic helix-loop-helix protein 6; Neurogenic basic-helix-loop-helix protein; Neurogenic differentiation factor 3) (Biotin); NEUROG1; Neurogenic differentiation factor 3; anti-NEUROG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NEUROG1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NeuroG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38~68 amino acids from the N-terminal region of human NeuroG1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-NeuroG1 N-term Pab is used in Western blot to detect NeuroG1 in A375 cell lysate.)

Related Product Information for anti-NEUROG1 antibody

Basic helix-loop-helix (bHLH) proteins are transcription factors involved in determining cell type during development. NeuroG1 is a bHLH protein with dual cell-fate specification roles. It functions during neurogenesis, and it has also been shown to inhibit the differentiation of neural stem cells into astrocytes. NeuroG1 promotes neurogenesis by functioning as a transcriptional activator, yet it inhibits astrocyte differentiation by compartmentalizing the CREB-binding protein transcription complex away from astrocyte differentiation genes and by inhibiting STAT transcription factors essential for gliogenesis.

Product Categories/Family for anti-NEUROG1 antibody

Antibodies; Transcription Factors

References

Tamimi, R.M., et al., Genomics 40(2):355-357 (1997). McCormick, M.B., et al., Mol. Cell. Biol. 16(10):5792-5800 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 38455396
• NCBI GeneID: 4762
• NCBI Accession #: NP_006152
• NCBI GenBank Nucleotide #: NM_006161.2
• UniProt Accession #: Q92886; Q5U0Q9; Q96HE1
• Molecular Weight: 25,718 Da
• NCBI Official Full Name: neurogenin-1
• NCBI Official Synonym Full Names: neurogenin 1
• NCBI Official Symbol: NEUROG1
• NCBI Official Synonym Symbols: AKA; ngn1; Math4C; bHLHa6; NEUROD3
• NCBI Protein Information: neurogenin-1
• UniProt Protein Name: Neurogenin-1
• Protein Family: Neurogenin
• UniProt Gene Name: NEUROG1
• UniProt Synonym Gene Names: BHLHA6; NEUROD3; NGN; NGN1; NGN-1; bHLHa6; NeuroD3
• UniProt Entry Name: NGN1_HUMAN

Uniprot Description

neurogenin 1: Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.

Protein type: Transcription factor; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 5q23-q31

Cellular Component: perikaryon; nucleus

Molecular Function: protein dimerization activity; DNA binding; chromatin binding; transcription factor activity

Biological Process: neuron differentiation; regulation of transcription from RNA polymerase II promoter; nervous system development; inner ear morphogenesis; cell fate commitment; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of neuron differentiation; positive regulation of exit from mitosis