Rabbit anti-Human, Mouse Neuritin Polyclonal Antibody | anti-NRN1 antibody

Neuritin Antibody

Cat. Num. AAA150407

• Gene Names: NRN1; NRN; dJ380B8.2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Neuritin Antibody is affinity chromatography purified via peptide column.
• Synonyms: Neuritin; Polyclonal Antibody; Neuritin Antibody; NRN; dJ380B8.2; neuritin 1; anti-NRN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Neuritin Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 142

• Applicable Applications for anti-NRN1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Neuritin antibody can be used for detection of Neuritin by Western blot at 5 and 10 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Neuritin antibody was raised against a 20 amino acid synthetic peptide from near the center of human neuritin.
• Buffer: Neuritin Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Neuritin antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of neuritin in Daudi cell lysate with neuritin antibody at (A) 5 and (B) 10 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Neuritin in mouse heart tissue with Neuritin antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Neuritin in Mouse Heart cells with Neuritin antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Neuritin in human brain tissue with Neuritin antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Neuritin in human brain tissue with Neuritin antibody at 20 μg/mL.Green: Neuritin Antibody (4101)Blue: DAPI staining)

Related Product Information for anti-NRN1 antibody

Neuritin Antibody: As the nervous system of a complex organism develops, it establishes functional networks through the growth and retraction of synaptic connections from growing axons and dendrites. This synaptic remodeling involves neuro-transmitter signaling, activation of neurotrophin receptors and alterations in gene expression. One such gene whose expression is increased by neural activity is neuritin, a GPI-anchored protein that is expressed in postmitotic differentiating neurons of the developing nervous system. Its expression is also induced by the neurotrophins BDNF and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic neuronal cultures, suggesting that neuritin may play a role as a downstream effector of activity-induced neurite outgrowth. More recent experiments have shown that neuritin is required for the androgen-induced axonal elongation in motor neurons and is upregulated following spinal cord injury, suggesting that neuritin may also play a role in survival and axonal regeneration.

NCBI and Uniprot Product Information

• NCBI GI #: 7706123
• NCBI GeneID: 51299
• NCBI Accession #: NP_057672
• NCBI GenBank Nucleotide #: NM_016588.2
• UniProt Accession #: Q9NPD7; Q7Z4Y1; B2RA93
• Molecular Weight: Predicted: 16 kDa; ; Observed: 15 kDa
• NCBI Official Full Name: neuritin isoform 1
• NCBI Official Synonym Full Names: neuritin 1
• NCBI Official Symbol: NRN1
• NCBI Official Synonym Symbols: NRN; dJ380B8.2
• NCBI Protein Information: neuritin
• UniProt Protein Name: Neuritin
• Protein Family: Neuritin
• UniProt Gene Name: NRN1
• UniProt Synonym Gene Names: NRN
• UniProt Entry Name: NRN1_HUMAN

NCBI Description

This gene encodes a member of the neuritin family, and is expressed in postmitotic-differentiating neurons of the developmental nervous system and neuronal structures associated with plasticity in the adult. The expression of this gene can be induced by neural activity and neurotrophins. The encoded protein contains a consensus cleavage signal found in glycosylphoshatidylinositol (GPI)-anchored proteins. The encoded protein promotes neurite outgrowth and arborization, suggesting its role in promoting neuritogenesis. Overexpression of the encoded protein may be associated with astrocytoma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

NRN1: Promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells. Belongs to the neuritin family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6p25.1

Cellular Component: plasma membrane; synapse; cell junction

Biological Process: nervous system development