Rabbit anti-Human, Mouse BCOR Polyclonal Antibody | anti-BCOR antibody

BCOR, ID (S1122) (BCL-6 Corepressor, BCoR, KIAA1575) (MaxLight 650)

Cat. Num. AAA6465127

• Gene Names: BCOR; MAA2; ANOP2; MCOPS2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: BCOR; Polyclonal Antibody; ID (S1122) (BCL-6 Corepressor; BCoR; KIAA1575) (MaxLight 650); anti-BCOR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BCOR. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
• Sequence Length: 1755

• Applicable Applications for anti-BCOR antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human BCOR (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BCOR Antibody (Center S1122) in 293 cell line lysates (35ug/lane). BCOR (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of BCOR Antibody (Center S1122) in mouse stomach tissue lysates (35ug/lane). BCOR (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(BCOR Antibody (Center S1122) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCOR Antibody (Center S1122) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(BCOR Antibody (Center S1122) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-BCOR antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Ghetu, A.F., Mol. Cell 29(3), 384-391 (2008). 2. Hilton, E.N., Hum. Mol. Genet. 16(14), 1773-1782 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 183396787
• NCBI GeneID: 54880
• NCBI Accession #: NP_001116857
• NCBI GenBank Nucleotide #: NP_001116857.1
• UniProt Accession #: Q6W2J9
• Molecular Weight: 192.189
• NCBI Official Full Name: BCL-6 corepressor isoform c
• NCBI Official Synonym Full Names: BCL6 corepressor
• NCBI Official Symbol: BCOR
• NCBI Official Synonym Symbols: MAA2; ANOP2; MCOPS2
• NCBI Protein Information: BCL-6 corepressor
• UniProt Protein Name: BCL-6 corepressor
• Protein Family: BCL-6 corepressor
• UniProt Gene Name: BCOR
• UniProt Synonym Gene Names: KIAA1575; BCoR
• UniProt Entry Name: BCOR_HUMAN

NCBI Description

The protein encoded by this gene was identified as an interacting corepressor of BCL6, a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6, but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein, which suggests a possible link between the two classes of HDACs. Several transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome Y.[provided by RefSeq, Jun 2010]

Uniprot Description

BCOR: Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2). Defects in BCOR are the cause of microphthalmia syndromic type 2 (MCOPS2). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS2 is a very rare multiple congenital anomaly syndrome characterized by eye anomalies (congenital cataract, microphthalmia, or secondary glaucoma), facial abnormalities (long narrow face, high nasal bridge, pointed nose with cartilages separated at the tip, cleft palate, or submucous cleft palate), cardiac anomalies (atrial septal defect, ventricular septal defect, or floppy mitral valve) and dental abnormalities (canine radiculomegaly, delayed dentition, oligodontia, persistent primary teeth, or variable root length). Belongs to the BCOR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: PcG protein complex; nucleus

Molecular Function: protein binding; heat shock protein binding; histone deacetylase binding; ubiquitin-protein ligase activity; transcription corepressor activity; transcription factor binding

Biological Process: odontogenesis; negative regulation of bone mineralization; negative regulation of histone H3-K36 methylation; transcription, DNA-dependent; heart development; specification of axis polarity; negative regulation of histone H3-K4 methylation; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent

Disease: Microphthalmia, Syndromic 2; Microphthalmia, Syndromic 1